TY - JOUR
T1 - Intraneuronal amyloid β42 enhanced by heating but counteracted by formic acid
AU - Ohyagi, Yasumasa
AU - Tsuruta, Yuko
AU - Motomura, Kyoko
AU - Miyoshi, Katsue
AU - Kikuchi, Hitoshi
AU - Iwaki, Toru
AU - Taniwaki, Takayuki
AU - Kira, Jun ichi
N1 - Funding Information:
We thank N. Suzuki (Takeda, Co.) for providing BC-05 and BA-27 antibodies. This work was supported by a Grant-in-Aid for Scientific Research by the Japan Ministry of Health, Welfare and Labor.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/1/15
Y1 - 2007/1/15
N2 - Amyloid β-protein ending at 42 (Aβ42) is the major peptide deposited in Alzheimer's disease (AD) brain. In immunocytochemical studies, formic acid treatment is used to dramatically enhance Aβ immunoreactivity. Recently, Aβ42 has been reported to accumulate in AD neurons. Since heating is known to enhance intracellular protein immunoreactivity, we used an autoclaving protocol to enhance intraneuronal Aβ42 immunoreactivity. Using this protocol, both anti-Aβ42 N-terminal and C-terminal antibodies, but not anti-Aβ40 C-terminal antibody, labeled AD neurons. Moreover, formic acid treatment counteracted such effects of autoclaving. Thus, intraneuronal Aβ42 accumulation may have been underestimated by conventional methods using formic acid only.
AB - Amyloid β-protein ending at 42 (Aβ42) is the major peptide deposited in Alzheimer's disease (AD) brain. In immunocytochemical studies, formic acid treatment is used to dramatically enhance Aβ immunoreactivity. Recently, Aβ42 has been reported to accumulate in AD neurons. Since heating is known to enhance intracellular protein immunoreactivity, we used an autoclaving protocol to enhance intraneuronal Aβ42 immunoreactivity. Using this protocol, both anti-Aβ42 N-terminal and C-terminal antibodies, but not anti-Aβ40 C-terminal antibody, labeled AD neurons. Moreover, formic acid treatment counteracted such effects of autoclaving. Thus, intraneuronal Aβ42 accumulation may have been underestimated by conventional methods using formic acid only.
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U2 - 10.1016/j.jneumeth.2006.06.010
DO - 10.1016/j.jneumeth.2006.06.010
M3 - Article
C2 - 16860394
AN - SCOPUS:33751248758
VL - 159
SP - 134
EP - 138
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
SN - 0165-0270
IS - 1
ER -