Abstract
Spinal microglia have been implicated in the pathogenesis of neuropathic pain after peripheral nerve injury concomitant with diseases such as diabetes and cancer. To reveal the etiological roles of microglia in behavioral pain hypersensitivity or neuronal excitability, technical approaches have been used. Here, we describe a technique for intrathecal transfer of cultured microglial cells through a catheter surgically implanted into the spinal subarachnoid space.
| Original language | English |
|---|---|
| Title of host publication | Microglia |
| Subtitle of host publication | Methods and Protocols |
| Pages | 291-294 |
| Number of pages | 4 |
| DOIs | |
| Publication status | Published - Jul 18 2013 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1041 |
| ISSN (Print) | 1064-3745 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
Cite this
Intrathecal infusion of microglia cells. / Masuda, Takahiro; Tsuda, Makoto; Tozaki-Saitoh, Hidetoshi; Inoue, Kazuhide.
Microglia: Methods and Protocols. 2013. p. 291-294 (Methods in Molecular Biology; Vol. 1041).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Intrathecal infusion of microglia cells
AU - Masuda, Takahiro
AU - Tsuda, Makoto
AU - Tozaki-Saitoh, Hidetoshi
AU - Inoue, Kazuhide
PY - 2013/7/18
Y1 - 2013/7/18
N2 - Spinal microglia have been implicated in the pathogenesis of neuropathic pain after peripheral nerve injury concomitant with diseases such as diabetes and cancer. To reveal the etiological roles of microglia in behavioral pain hypersensitivity or neuronal excitability, technical approaches have been used. Here, we describe a technique for intrathecal transfer of cultured microglial cells through a catheter surgically implanted into the spinal subarachnoid space.
AB - Spinal microglia have been implicated in the pathogenesis of neuropathic pain after peripheral nerve injury concomitant with diseases such as diabetes and cancer. To reveal the etiological roles of microglia in behavioral pain hypersensitivity or neuronal excitability, technical approaches have been used. Here, we describe a technique for intrathecal transfer of cultured microglial cells through a catheter surgically implanted into the spinal subarachnoid space.
UR - http://www.scopus.com/inward/record.url?scp=84880110982&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880110982&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-520-0_25
DO - 10.1007/978-1-62703-520-0_25
M3 - Chapter
C2 - 23813387
AN - SCOPUS:84880110982
SN - 9781627035194
T3 - Methods in Molecular Biology
SP - 291
EP - 294
BT - Microglia
ER -