Intratumoral injection of immature dendritic cells enhances antitumor effect of hyperthermia using magnetic nanoparticles

Kouji Tanaka, Akira Ito, Takeshi Kobayashi, Tatsuyoshi Kawamura, Shinji Shimada, Kazuhiko Matsumoto, Toshiaki Saida, Hiroyuki Honda

Research output: Contribution to journalArticle

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Abstract

Dendritic cells (DCs) are potent antigen-presenting cells that play a pivotal role in regulating immune responses in cancer and have recently been shown to be activated by heat shock proteins (HSPs). We previously reported that HSP70 expression after hyperthermia induces antitumor immunity. Our hyperthermia system using magnetite cationic liposomes (MCLs) induced necrotic cell death that was correlated with HSP70 release. In the present study, we investigated the therapeutic effects of DC therapy combined with MCL-induced hyperthermia on mouse melanoma. In an in vitro study, when immature DCs were pulsed with mouse B16 melanoma cells heated at 43°C, major histocompatibility complex (MHC) class I/II, costimulatory molecules CD80/ CD86 and CCR7 in the DCs were upregulated, thus resulting in DC maturation. C57BL/6 mice bearing a melanoma nodule were subjected to combination therapy using hyperthermia and DC immunotherapy in vivo by means of tumor-specific hyperthermia using MCLs and directly injected immature DCs. Mice were divided into 4 groups: group I (control), group II (hyperthermia), group III (DC therapy) and group IV (hyperthermia + DC therapy). Complete regression of tumors was observed in 60% of mice in group IV, while no tumor regression was seen among mice in the other groups. Increased cytotoxic T lymphocyte (CTL) and natural killer (NK) activity was observed on in vitro cytotoxicity assay using splenocytes in the cured mice treated with combination therapy, and the cured mice rejected a second challenge of B16 melanoma cells. This study has important implications for the application of MCL-induced hyperthermia plus DC therapy in patients with advanced malignancies as a novel cancer therapy.

Original languageEnglish
Pages (from-to)624-633
Number of pages10
JournalInternational Journal of Cancer
Volume116
Issue number4
DOIs
Publication statusPublished - Sep 10 2005

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Nanoparticles
Dendritic Cells
Fever
Injections
Ferrosoferric Oxide
Cell- and Tissue-Based Therapy
Liposomes
Neoplasms
Experimental Melanomas
Induced Hyperthermia
Melanoma
Cytotoxic T-Lymphocytes
Therapeutic Uses
Antigen-Presenting Cells
Heat-Shock Proteins
Major Histocompatibility Complex
Inbred C57BL Mouse
Immunotherapy
Immunity
Cell Death

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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Intratumoral injection of immature dendritic cells enhances antitumor effect of hyperthermia using magnetic nanoparticles. / Tanaka, Kouji; Ito, Akira; Kobayashi, Takeshi; Kawamura, Tatsuyoshi; Shimada, Shinji; Matsumoto, Kazuhiko; Saida, Toshiaki; Honda, Hiroyuki.

In: International Journal of Cancer, Vol. 116, No. 4, 10.09.2005, p. 624-633.

Research output: Contribution to journalArticle

Tanaka, Kouji ; Ito, Akira ; Kobayashi, Takeshi ; Kawamura, Tatsuyoshi ; Shimada, Shinji ; Matsumoto, Kazuhiko ; Saida, Toshiaki ; Honda, Hiroyuki. / Intratumoral injection of immature dendritic cells enhances antitumor effect of hyperthermia using magnetic nanoparticles. In: International Journal of Cancer. 2005 ; Vol. 116, No. 4. pp. 624-633.
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