Intrinsic inhibitor of inositol 1,4,5-trisphosphate binding

Masato Hirata, Masako Yoshida, Takashi Kanematsu, Hiroshi Takeuchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Rat brain cytosol was applied to a heparin column and eluted with 0.9 M-NaCl. The total binding activity of [3H] inositol 1,4,5-trisphosphate to the eluate was increased about 6-fold compared with the original cytosol. When the eluate was mixed with a flow-through fraction from the heparin column, however, the activity returned to the original lever, suggesting that the flow-through fraction contained an inhibitory factor(s) which prevented the binding. The factor(s) was purified by sequential column chromatography using gel permeation, a hydrophobic gel, and finally, a hydroxylapatite gel. Silver staining of sodium dedecyl sulfate gel electrphoresis of the sample thus purified showed a broad band located between the authentic molecular weight markers of 580 and 390 k. A carbohydrate staining method showed that the factor is a glycoprotein.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume190
Issue number1-2
Publication statusPublished - 1999

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Inositol 1,4,5-Trisphosphate
Gels
Cytosol
Heparin
Silver Staining
Durapatite
Column chromatography
Gel Chromatography
Glycoproteins
Molecular Weight
Silver
Carbohydrates
Permeation
Staining and Labeling
Rats
Brain
Molecular weight

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Intrinsic inhibitor of inositol 1,4,5-trisphosphate binding. / Hirata, Masato; Yoshida, Masako; Kanematsu, Takashi; Takeuchi, Hiroshi.

In: Molecular and Cellular Biochemistry, Vol. 190, No. 1-2, 1999, p. 179-184.

Research output: Contribution to journalArticle

Hirata, M, Yoshida, M, Kanematsu, T & Takeuchi, H 1999, 'Intrinsic inhibitor of inositol 1,4,5-trisphosphate binding', Molecular and Cellular Biochemistry, vol. 190, no. 1-2, pp. 179-184.
Hirata, Masato ; Yoshida, Masako ; Kanematsu, Takashi ; Takeuchi, Hiroshi. / Intrinsic inhibitor of inositol 1,4,5-trisphosphate binding. In: Molecular and Cellular Biochemistry. 1999 ; Vol. 190, No. 1-2. pp. 179-184.
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