Introduction of a specific binding domain on myoglobin surface by new chemical modification

Takashi Hayashi, Tsutomu Ando, Takaaki Matsuda, Hiroaki Yonemura, Sunao Yamada, Yoshio Hisaeda

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A new myoglobin, reconstituted with a modified zinc protoporphyrin, having a total of four ammonium groups at the terminal of the two propionate side chains was constructed to introduce a substrate binding site. The protein with a positively charged patch on the surface formed a stable complex with negatively charged substrates, such as hexacyanoferrate(III) and anthraquinonesulfonate via an electrostatic interaction. The complexation was monitored by fluorescence quenching due to singlet electron transfer from the photoexcited reconstituted zinc myoglobin to the substrates. The binding properties were evaluated by Stern-Volmer plots from the fluorescence quenching of the zinc myoglobin by a quencher. Particularly, anthraquinone-2,7-disulfonic acid showed a high affinity with a binding constant of 1.5x105 M-1 in 10 mM phosphate buffer, pH 7.0. In contrast, the plots upon the addition of anthraquinone-2-sulfonic acid at different ionic strengths indicated that the complex was formed not only by an electrostatic interaction but also by a hydrophobic contact. The findings from the fluorescence studies conclude that the present system is a useful model for discussion of electron transfer via non-covalently linked donor-acceptor pairing on the protein surface. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalJournal of Inorganic Biochemistry
Volume82
Issue number1-4
DOIs
Publication statusPublished - Nov 30 2000

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Myoglobin
Chemical modification
Fluorescence
Coulomb interactions
Static Electricity
Zinc
Quenching
Substrates
Electrons
Anthraquinones
Propionates
Ionic strength
Complexation
Ammonium Compounds
Osmolar Concentration
Buffers
Membrane Proteins
Phosphates
Binding Sites
Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Inorganic Chemistry

Cite this

Introduction of a specific binding domain on myoglobin surface by new chemical modification. / Hayashi, Takashi; Ando, Tsutomu; Matsuda, Takaaki; Yonemura, Hiroaki; Yamada, Sunao; Hisaeda, Yoshio.

In: Journal of Inorganic Biochemistry, Vol. 82, No. 1-4, 30.11.2000, p. 133-139.

Research output: Contribution to journalArticle

Hayashi, Takashi ; Ando, Tsutomu ; Matsuda, Takaaki ; Yonemura, Hiroaki ; Yamada, Sunao ; Hisaeda, Yoshio. / Introduction of a specific binding domain on myoglobin surface by new chemical modification. In: Journal of Inorganic Biochemistry. 2000 ; Vol. 82, No. 1-4. pp. 133-139.
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