Introduction of ID2 enhances invasiveness in ID2-null oral squamous cell carcinoma cells via the SNAIL axis

Yu Kamata, Tomoki Sumida, Yosuke Kobayashi, Akiko Ishikawa, Kumamaru Wataru, Yoshihide Mori

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aim: Inhibitor of DNA-binding (ID) proteins are negative regulators of basic helix-loop-helix transcription factors that generally stimulate cell proliferation and inhibit differentiation. However, the role of ID2 in cancer progression remains ambiguous. Here, we investigated the function of ID2 in ID2-null oral squamous cell carcinoma (OSCC) cells. Materials and Methods: We introduced an ID2 cDNA construct into ID2-null OSCC cells and compared them with empty-vector-transfected cells in terms of cell proliferation, invasion, and activity and expression of matrix metalloproteinase (MMP). Results: ID2 introduction resulted in enhanced malignant phenotypes. The ID2-expressing cells showed increased N-cadherin, vimentin, and E-cadherin expression and epithelial-mesenchymal transition. In addition, cell invasion drastically increased with increased expression and activity of MMP2. Immunoprecipitation revealed a direct interaction between ID2 and zinc finger transcription factor, snail family transcriptional repressor 1 (SNAIL1). Conclusion: ID2 expression triggered a malignant phenotype, especially of invasive properties, through the ID2- SNAIL axis. Thus, ID2 represents a potential therapeutic target for OSCC.

Original languageEnglish
Pages (from-to)493-498
Number of pages6
JournalCancer Genomics and Proteomics
Volume13
Issue number6
DOIs
Publication statusPublished - Nov 1 2016

Fingerprint

Squamous Cell Carcinoma
Cells
Cell proliferation
Cadherins
Basic Helix-Loop-Helix Transcription Factors
DNA-Binding Proteins
Vimentin
Matrix Metalloproteinases
Cell Proliferation
Zinc
Transcription Factors
Phenotype
Complementary DNA
Epithelial-Mesenchymal Transition
Zinc Fingers
Immunoprecipitation
Epithelial Cells
Neoplasms
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Introduction of ID2 enhances invasiveness in ID2-null oral squamous cell carcinoma cells via the SNAIL axis. / Kamata, Yu; Sumida, Tomoki; Kobayashi, Yosuke; Ishikawa, Akiko; Wataru, Kumamaru; Mori, Yoshihide.

In: Cancer Genomics and Proteomics, Vol. 13, No. 6, 01.11.2016, p. 493-498.

Research output: Contribution to journalArticle

@article{cc291d426e2a45669c2eb75e61b1e949,
title = "Introduction of ID2 enhances invasiveness in ID2-null oral squamous cell carcinoma cells via the SNAIL axis",
abstract = "Aim: Inhibitor of DNA-binding (ID) proteins are negative regulators of basic helix-loop-helix transcription factors that generally stimulate cell proliferation and inhibit differentiation. However, the role of ID2 in cancer progression remains ambiguous. Here, we investigated the function of ID2 in ID2-null oral squamous cell carcinoma (OSCC) cells. Materials and Methods: We introduced an ID2 cDNA construct into ID2-null OSCC cells and compared them with empty-vector-transfected cells in terms of cell proliferation, invasion, and activity and expression of matrix metalloproteinase (MMP). Results: ID2 introduction resulted in enhanced malignant phenotypes. The ID2-expressing cells showed increased N-cadherin, vimentin, and E-cadherin expression and epithelial-mesenchymal transition. In addition, cell invasion drastically increased with increased expression and activity of MMP2. Immunoprecipitation revealed a direct interaction between ID2 and zinc finger transcription factor, snail family transcriptional repressor 1 (SNAIL1). Conclusion: ID2 expression triggered a malignant phenotype, especially of invasive properties, through the ID2- SNAIL axis. Thus, ID2 represents a potential therapeutic target for OSCC.",
author = "Yu Kamata and Tomoki Sumida and Yosuke Kobayashi and Akiko Ishikawa and Kumamaru Wataru and Yoshihide Mori",
year = "2016",
month = "11",
day = "1",
doi = "10.21873/cgp.20012",
language = "English",
volume = "13",
pages = "493--498",
journal = "Cancer Genomics and Proteomics",
issn = "1109-6535",
publisher = "International Institute of Anticancer Research",
number = "6",

}

TY - JOUR

T1 - Introduction of ID2 enhances invasiveness in ID2-null oral squamous cell carcinoma cells via the SNAIL axis

AU - Kamata, Yu

AU - Sumida, Tomoki

AU - Kobayashi, Yosuke

AU - Ishikawa, Akiko

AU - Wataru, Kumamaru

AU - Mori, Yoshihide

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Aim: Inhibitor of DNA-binding (ID) proteins are negative regulators of basic helix-loop-helix transcription factors that generally stimulate cell proliferation and inhibit differentiation. However, the role of ID2 in cancer progression remains ambiguous. Here, we investigated the function of ID2 in ID2-null oral squamous cell carcinoma (OSCC) cells. Materials and Methods: We introduced an ID2 cDNA construct into ID2-null OSCC cells and compared them with empty-vector-transfected cells in terms of cell proliferation, invasion, and activity and expression of matrix metalloproteinase (MMP). Results: ID2 introduction resulted in enhanced malignant phenotypes. The ID2-expressing cells showed increased N-cadherin, vimentin, and E-cadherin expression and epithelial-mesenchymal transition. In addition, cell invasion drastically increased with increased expression and activity of MMP2. Immunoprecipitation revealed a direct interaction between ID2 and zinc finger transcription factor, snail family transcriptional repressor 1 (SNAIL1). Conclusion: ID2 expression triggered a malignant phenotype, especially of invasive properties, through the ID2- SNAIL axis. Thus, ID2 represents a potential therapeutic target for OSCC.

AB - Aim: Inhibitor of DNA-binding (ID) proteins are negative regulators of basic helix-loop-helix transcription factors that generally stimulate cell proliferation and inhibit differentiation. However, the role of ID2 in cancer progression remains ambiguous. Here, we investigated the function of ID2 in ID2-null oral squamous cell carcinoma (OSCC) cells. Materials and Methods: We introduced an ID2 cDNA construct into ID2-null OSCC cells and compared them with empty-vector-transfected cells in terms of cell proliferation, invasion, and activity and expression of matrix metalloproteinase (MMP). Results: ID2 introduction resulted in enhanced malignant phenotypes. The ID2-expressing cells showed increased N-cadherin, vimentin, and E-cadherin expression and epithelial-mesenchymal transition. In addition, cell invasion drastically increased with increased expression and activity of MMP2. Immunoprecipitation revealed a direct interaction between ID2 and zinc finger transcription factor, snail family transcriptional repressor 1 (SNAIL1). Conclusion: ID2 expression triggered a malignant phenotype, especially of invasive properties, through the ID2- SNAIL axis. Thus, ID2 represents a potential therapeutic target for OSCC.

UR - http://www.scopus.com/inward/record.url?scp=84994351239&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994351239&partnerID=8YFLogxK

U2 - 10.21873/cgp.20012

DO - 10.21873/cgp.20012

M3 - Article

C2 - 27807072

AN - SCOPUS:84994351239

VL - 13

SP - 493

EP - 498

JO - Cancer Genomics and Proteomics

JF - Cancer Genomics and Proteomics

SN - 1109-6535

IS - 6

ER -