Introduction of the hiC6 gene, which encodes a homologue of a late embryogenesis abundant (LEA) protein, enhances freezing tolerance of yeast

Ken-ichi Honjoh, Yuichi Oda, Ryoji Takata, Takahisa Miyamoto, Shoji Hatano

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The hiC6 gene of Chlorella vulgaris, sharing sequence similarity with a late embryogenesis abundant (lea) gene, was introduced into Saccharomyces cerevisiae. It was inserted on a multicopy plasmid under the transcriptional control of the yeast GAL1 promoter. Expression of HIC6 protein was confirmed by immunochemical methods. Expression level of the protein was increased gradually with the induction-time by galactose. With maximum induction time, the freeze-tolerance of yeast transformed with hiC6 gene was approximately 3.3 times (from 20% to 66% survival rate) higher than that of the control yeast.

Original languageEnglish
Pages (from-to)509-512
Number of pages4
JournalJournal of Plant Physiology
Volume155
Issue number4-5
DOIs
Publication statusPublished - Oct 1999

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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