Intron retention generates a novel isoform of CEACAM6 that may act as an adhesion molecule in the ectoplasmic specialization structures between spermatids and sertoli cells in rat testis

Hitoshi Kurio, Emi Murayama, Takane Kaneko, Yosaburo Shibata, Tetsuichiro Inai, Hiroshi Iida

Research output: Contribution to journalArticle

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Abstract

By differential display technique followed by RT-PCR and DNA sequence analyses, we isolated carcinoembryonic antigen-related cell adhesion molecule 6 (Ceacam6) and its novel spliced variant Ceacam6-Long (Ceacam6-L) from rat testis. Ceacam6-L mRNA was generated by retention of 67 nucleotide-length third intron in Ceacam6 gene. Ceacam6-L is a member of an immunoglobulin superfamily and encodes a protein of 50 kDa with a signal sequence at the N-terminus, one immunoglobulin (Ig)-like domain, three IgCAM domains, a transmembrane region, and a short intracellular region. Expression analyses by RT-PCR and Northern blot showed that Ceacam6-L was exclusively expressed in rat testis and first detectable at 5 wk during postnatal development of testis. We performed immunoblot analyses and immunohistochemistry using the anti-CEACAM6-L antibody. Confocal laser scanning microscopy revealed that CEACAM6-L was not present at blood-testis barrier junctions between Sertoli cells but localized at the interface between Sertoli cells and germ cells, possibly to work as an adhesion molecule in the apical compartment of the seminiferous epithelium. At stages VII-VIII, at which all of the elongated spermatids migrated to the luminal surface of the seminiferous tubules, CEACAM6-L was found to locate at the concave side of elongated spermatid heads, following the curvature of their sickle-shaped nuclei, suggesting that CEACAM6-L might be involved in the anchoring of spermatids to Sertoli cells and spermiation. We concluded that CEACAM6-L might be a novel adhesion molecule constructing the apical ectoplasmic specialization in testis.

Original languageEnglish
Pages (from-to)1062-1073
Number of pages12
JournalBiology of Reproduction
Volume79
Issue number6
DOIs
Publication statusPublished - Dec 1 2008

Fingerprint

Spermatids
Sertoli Cells
Carcinoembryonic Antigen
Cell Adhesion Molecules
Introns
Testis
Protein Isoforms
Blood-Testis Barrier
Seminiferous Epithelium
Polymerase Chain Reaction
Seminiferous Tubules
Protein Sorting Signals
DNA Sequence Analysis
Germ Cells
Confocal Microscopy
Northern Blotting
Immunoglobulins
Nucleotides
Immunohistochemistry
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Intron retention generates a novel isoform of CEACAM6 that may act as an adhesion molecule in the ectoplasmic specialization structures between spermatids and sertoli cells in rat testis. / Kurio, Hitoshi; Murayama, Emi; Kaneko, Takane; Shibata, Yosaburo; Inai, Tetsuichiro; Iida, Hiroshi.

In: Biology of Reproduction, Vol. 79, No. 6, 01.12.2008, p. 1062-1073.

Research output: Contribution to journalArticle

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