Invariant natural killer T cells play dual roles in the development of experimental autoimmune uveoretinitis

Masashi Satoh, Ken ichi Namba, Nobuyoshi Kitaichi, Noriko Endo, Hirokuni Kitamei, Daiju Iwata, Shigeaki Ohno, Susumu Ishida, Kazunori Onoé, Hiroshi Watarai, Masaru Taniguchi, Tatsuro Ishibashi, Joan Stein-Streilein, Kohei Sonoda, Luc Van Kaer, Kazuya Iwabuchi

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Abstract

Experimental autoimmune uveoretinitis (EAU) represents an experimental model for human endogenous uveitis, which is caused by Th1/Th17 cell-mediated inflammation. Natural killer T (NKT) cells recognize lipid antigens and produce large amounts of cytokines upon activation. To examine the role of NKT cells in the development of uveitis, EAU was elicited by immunization with a peptide from the human interphotoreceptor retinoid-binding protein (hIRBP1-20) in complete Freund's adjuvant and histopathology scores were evaluated in C57BL/6 (WT) and NKT cell-deficient mice. NKT cell-deficient mice developed more severe EAU pathology than WT mice. When WT mice were treated with ligands of the invariant subset of NKT cells (α-GalCer or RCAI-56), EAU was ameliorated in mice treated with RCAI-56 but not α-GalCer. IRBP-specific Th1/Th17 cytokines were reduced in RCAI-56-treated compared with vehicle-treated mice. Although the numbers of IRBP-specific T cells detected by hIRBP3-13/I-Ab tetramers in the spleen and the draining lymph node were the same for vehicle and RCAI-56 treatment groups, RORγt expression by tetramer-positive cells in RCAI-56-treated mice was lower than in control mice. Moreover, the eyes of RCAI-56-treated mice contained fewer IRBP-specific T cells compared with control mice. These results suggest that invariant NKT (iNKT) cells suppress the induction of Th17 cells and infiltration of IRBP-specific T cells into the eyes, thereby reducing ocular inflammation. However, in sharp contrast to the ameliorating effects of iNKT cell activation during the initiation phase of EAU, iNKT cell activation during the effector phase exacerbated disease pathology. Thus, we conclude that iNKT cells exhibit dual roles in the development of EAU.

Original languageEnglish
Pages (from-to)79-89
Number of pages11
JournalExperimental Eye Research
Volume153
DOIs
Publication statusPublished - Dec 1 2016

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Natural Killer T-Cells
Th17 Cells
Uveitis
T-Lymphocytes
Pathology
Cytokines
Inflammation
Th1 Cells
Freund's Adjuvant
Immunization
Theoretical Models
Spleen
Lymph Nodes
Ligands
Lipids
Antigens
Peptides

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Invariant natural killer T cells play dual roles in the development of experimental autoimmune uveoretinitis. / Satoh, Masashi; Namba, Ken ichi; Kitaichi, Nobuyoshi; Endo, Noriko; Kitamei, Hirokuni; Iwata, Daiju; Ohno, Shigeaki; Ishida, Susumu; Onoé, Kazunori; Watarai, Hiroshi; Taniguchi, Masaru; Ishibashi, Tatsuro; Stein-Streilein, Joan; Sonoda, Kohei; Van Kaer, Luc; Iwabuchi, Kazuya.

In: Experimental Eye Research, Vol. 153, 01.12.2016, p. 79-89.

Research output: Contribution to journalArticle

Satoh, M, Namba, KI, Kitaichi, N, Endo, N, Kitamei, H, Iwata, D, Ohno, S, Ishida, S, Onoé, K, Watarai, H, Taniguchi, M, Ishibashi, T, Stein-Streilein, J, Sonoda, K, Van Kaer, L & Iwabuchi, K 2016, 'Invariant natural killer T cells play dual roles in the development of experimental autoimmune uveoretinitis', Experimental Eye Research, vol. 153, pp. 79-89. https://doi.org/10.1016/j.exer.2016.10.003
Satoh, Masashi ; Namba, Ken ichi ; Kitaichi, Nobuyoshi ; Endo, Noriko ; Kitamei, Hirokuni ; Iwata, Daiju ; Ohno, Shigeaki ; Ishida, Susumu ; Onoé, Kazunori ; Watarai, Hiroshi ; Taniguchi, Masaru ; Ishibashi, Tatsuro ; Stein-Streilein, Joan ; Sonoda, Kohei ; Van Kaer, Luc ; Iwabuchi, Kazuya. / Invariant natural killer T cells play dual roles in the development of experimental autoimmune uveoretinitis. In: Experimental Eye Research. 2016 ; Vol. 153. pp. 79-89.
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AU - Kitamei, Hirokuni

AU - Iwata, Daiju

AU - Ohno, Shigeaki

AU - Ishida, Susumu

AU - Onoé, Kazunori

AU - Watarai, Hiroshi

AU - Taniguchi, Masaru

AU - Ishibashi, Tatsuro

AU - Stein-Streilein, Joan

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AU - Van Kaer, Luc

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