Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo

Shinsuke Fujii, Hidefumi Maeda, Naohisa Wada, Atsushi Tomokiyo, Masahiro Saito, Akifumi Akamine

Research output: Contribution to journalArticle

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Abstract

The lifespan of the tooth is influenced by the periodontal ligament (PDL), a specialized connective tissue that connects the cementum with the tooth socket bone. Generation of a cell line from PDL progenitor/stem cells would allow development of tissue engineering-based regenerative PDL therapy. However, little is known about the characteristics of PDL progenitor/stem cells because PDL tissue consists of a heterogeneous cell population and there are no pure PDL cell lines. Recently, we succeeded in immortalizing primary human PDL fibroblasts (HPLFs) by transfecting them with SV40 T-antigen and hTERT (Cell Tissue Res 2006; 324: 117-125). In this study, we isolated three clonal cell lines from these immortalized cells (lines 1-4, 1-11, and 1-24) that express RUNX-2, Col1, ALP, OPN, OCN, RANKL, OPG, scleraxis, periostin, Col XII, and α-SMA mRNA. Immunocytochemical analysis demonstrated that CD146 was expressed in cell lines 1-4 and 1-11 and that STRO-1 was expressed in lines 1-11 and 1-24. Lines 1-4 and 1-11 differentiated into osteoblastic cells and adipocytes when cultured in lineage-specific differentiation media. Four weeks after transplanting cell line 1-11 into immunodeficient mice with β-tricalcium phosphate (β-TCP), the transplant produced cementum/bone-like tissues around the β-TCP. Eight weeks after transplantation, the 1-11 celltransplantformed PDL-like structures on the surface of the β-TCP. These data suggest that cell line 1-11 was derived from a progenitor/stem cell present in the PDL and should be very useful for studying the biology and regeneration of human periodontium.

Original languageEnglish
Pages (from-to)743-749
Number of pages7
JournalJournal of cellular physiology
Volume215
Issue number3
DOIs
Publication statusPublished - Jun 1 2008

Fingerprint

Periodontal Ligament
Ligaments
Stem cells
Stem Cells
Cell Line
Cells
Tissue
Dental Cementum
Bone
Tooth Socket
Polyomavirus Transforming Antigens
In Vitro Techniques
Bone and Bones
Periodontium
Transplants
Fibroblasts
Tissue Engineering
Tissue engineering
Adipocytes
Connective Tissue

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo. / Fujii, Shinsuke; Maeda, Hidefumi; Wada, Naohisa; Tomokiyo, Atsushi; Saito, Masahiro; Akamine, Akifumi.

In: Journal of cellular physiology, Vol. 215, No. 3, 01.06.2008, p. 743-749.

Research output: Contribution to journalArticle

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