Investigation of optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture

K. Nakazawa, H. Ijima, M. Kaneko, T. Ito, T. Matsushita, T. Gion, K. Shirabe, M. Shimada, K. Takenaka, K. Sugimachi, K. Funatsu

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

In this study, we determined optimum culture conditions of primary porcine hepatocytes for development of hybrid artificial liver using polyurethane foam (PUF)/spheroid culture. Optimum components of culture medium were investigated in stationary culture using PUF plate (25 x 25 x 1 mm). Immobilized cell density and intracapillary medium velocity in multi- capillary (MC) PUF packed-bed module (volume: 18.8 cm3) were estimated in perfusion culture. As a result, porcine hepatocytes formed spheroid spontaneously and maintained ammonia metabolic activity in Williams'E medium supplemented with 10 to 20% FBS. And high activities of ammonia metabolism and albumin secretion were performed at the immobilized cell density. This study provided optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalJapanese Journal of Artificial Organs
Volume27
Issue number1
Publication statusPublished - Jan 1 1998

All Science Journal Classification (ASJC) codes

  • Biophysics

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