Investigation of optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture

K. Nakazawa, H. Ijima, M. Kaneko, T. Ito, T. Matsushita, T. Gion, K. Shirabe, M. Shimada, K. Takenaka, K. Sugimachi, K. Funatsu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, we determined optimum culture conditions of primary porcine hepatocytes for development of hybrid artificial liver using polyurethane foam (PUF)/spheroid culture. Optimum components of culture medium were investigated in stationary culture using PUF plate (25 x 25 x 1 mm). Immobilized cell density and intracapillary medium velocity in multi- capillary (MC) PUF packed-bed module (volume: 18.8 cm3) were estimated in perfusion culture. As a result, porcine hepatocytes formed spheroid spontaneously and maintained ammonia metabolic activity in Williams'E medium supplemented with 10 to 20% FBS. And high activities of ammonia metabolism and albumin secretion were performed at the immobilized cell density. This study provided optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalJapanese Journal of Artificial Organs
Volume27
Issue number1
Publication statusPublished - Jan 1 1998

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Artificial Liver
Hepatocytes
Swine
Immobilized Cells
Ammonia
Cell Count
Culture Media
Albumins
Perfusion
polyurethane foam

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

Investigation of optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture. / Nakazawa, K.; Ijima, H.; Kaneko, M.; Ito, T.; Matsushita, T.; Gion, T.; Shirabe, K.; Shimada, M.; Takenaka, K.; Sugimachi, K.; Funatsu, K.

In: Japanese Journal of Artificial Organs, Vol. 27, No. 1, 01.01.1998, p. 222-226.

Research output: Contribution to journalArticle

Nakazawa, K, Ijima, H, Kaneko, M, Ito, T, Matsushita, T, Gion, T, Shirabe, K, Shimada, M, Takenaka, K, Sugimachi, K & Funatsu, K 1998, 'Investigation of optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture', Japanese Journal of Artificial Organs, vol. 27, no. 1, pp. 222-226.
Nakazawa, K. ; Ijima, H. ; Kaneko, M. ; Ito, T. ; Matsushita, T. ; Gion, T. ; Shirabe, K. ; Shimada, M. ; Takenaka, K. ; Sugimachi, K. ; Funatsu, K. / Investigation of optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture. In: Japanese Journal of Artificial Organs. 1998 ; Vol. 27, No. 1. pp. 222-226.
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