Investigation of routes of Salmonella contamination via soils and the use of mulch for contamination control during lettuce cultivation

Ken-ichi Honjoh, Tomoko Mishima, Nozomi Kido, Misako Shimamoto, Takahisa Miyamoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Foodborne illnesses associated with the consumption of fresh produce such as raw vegetables have become a major health concern worldwide in recent years. In the present study, we investigated the possible routes of Salmonella contamination in leafy lettuce via soil during cultivation. After 10-week cultivation of lettuce plants in soils inoculated with S. Enteritidis expressing green fluorescent protein (SE-EGFP), the bacterium was detected in soil inoculated with >104 cfu/g and from most lettuce leaves cultivated in soils inoculated with >4.4 × 107 cfu/ g. As Salmonella was not detected in intact lettuce leaves or lettuce leaves with root injury cultivated in highly contaminated soils and after surface disinfection, the lettuce plants were not considered to internalize the bacterium. Overhead irrigation led to the contamination of one in 10 lettuce plants; however, all sets of three leaves of the plant were contaminated (>110 MPN/g). In an effort to prevent Salmonella contamination from soils, we investigated the effects of mulch on contamination levels during cultivation. Mulch effectively reduced Salmonella contamination levels of lettuce plants cultivated in highly contaminated soils.

Original languageEnglish
Pages (from-to)961-969
Number of pages9
JournalFood Science and Technology Research
Volume20
Issue number5
DOIs
Publication statusPublished - Jan 1 2014

Fingerprint

Lettuce
Salmonella
soil pollution
lettuce
Contamination
Soil
leaf lettuce
Soils
polluted soils
soil
overhead irrigation
Bacteria
fresh produce
raw vegetables
bacteria
Salmonella Enteritidis
disinfection
foodborne illness
green fluorescent protein
tillage

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

Cite this

Investigation of routes of Salmonella contamination via soils and the use of mulch for contamination control during lettuce cultivation. / Honjoh, Ken-ichi; Mishima, Tomoko; Kido, Nozomi; Shimamoto, Misako; Miyamoto, Takahisa.

In: Food Science and Technology Research, Vol. 20, No. 5, 01.01.2014, p. 961-969.

Research output: Contribution to journalArticle

@article{b6d1e93877314a039e1caf8b56a1092d,
title = "Investigation of routes of Salmonella contamination via soils and the use of mulch for contamination control during lettuce cultivation",
abstract = "Foodborne illnesses associated with the consumption of fresh produce such as raw vegetables have become a major health concern worldwide in recent years. In the present study, we investigated the possible routes of Salmonella contamination in leafy lettuce via soil during cultivation. After 10-week cultivation of lettuce plants in soils inoculated with S. Enteritidis expressing green fluorescent protein (SE-EGFP), the bacterium was detected in soil inoculated with >104 cfu/g and from most lettuce leaves cultivated in soils inoculated with >4.4 × 107 cfu/ g. As Salmonella was not detected in intact lettuce leaves or lettuce leaves with root injury cultivated in highly contaminated soils and after surface disinfection, the lettuce plants were not considered to internalize the bacterium. Overhead irrigation led to the contamination of one in 10 lettuce plants; however, all sets of three leaves of the plant were contaminated (>110 MPN/g). In an effort to prevent Salmonella contamination from soils, we investigated the effects of mulch on contamination levels during cultivation. Mulch effectively reduced Salmonella contamination levels of lettuce plants cultivated in highly contaminated soils.",
author = "Ken-ichi Honjoh and Tomoko Mishima and Nozomi Kido and Misako Shimamoto and Takahisa Miyamoto",
year = "2014",
month = "1",
day = "1",
doi = "10.3136/fstr.20.961",
language = "English",
volume = "20",
pages = "961--969",
journal = "Food Science and Technology Research",
issn = "1344-6606",
publisher = "Japanese Society for Food Science and Technology",
number = "5",

}

TY - JOUR

T1 - Investigation of routes of Salmonella contamination via soils and the use of mulch for contamination control during lettuce cultivation

AU - Honjoh, Ken-ichi

AU - Mishima, Tomoko

AU - Kido, Nozomi

AU - Shimamoto, Misako

AU - Miyamoto, Takahisa

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Foodborne illnesses associated with the consumption of fresh produce such as raw vegetables have become a major health concern worldwide in recent years. In the present study, we investigated the possible routes of Salmonella contamination in leafy lettuce via soil during cultivation. After 10-week cultivation of lettuce plants in soils inoculated with S. Enteritidis expressing green fluorescent protein (SE-EGFP), the bacterium was detected in soil inoculated with >104 cfu/g and from most lettuce leaves cultivated in soils inoculated with >4.4 × 107 cfu/ g. As Salmonella was not detected in intact lettuce leaves or lettuce leaves with root injury cultivated in highly contaminated soils and after surface disinfection, the lettuce plants were not considered to internalize the bacterium. Overhead irrigation led to the contamination of one in 10 lettuce plants; however, all sets of three leaves of the plant were contaminated (>110 MPN/g). In an effort to prevent Salmonella contamination from soils, we investigated the effects of mulch on contamination levels during cultivation. Mulch effectively reduced Salmonella contamination levels of lettuce plants cultivated in highly contaminated soils.

AB - Foodborne illnesses associated with the consumption of fresh produce such as raw vegetables have become a major health concern worldwide in recent years. In the present study, we investigated the possible routes of Salmonella contamination in leafy lettuce via soil during cultivation. After 10-week cultivation of lettuce plants in soils inoculated with S. Enteritidis expressing green fluorescent protein (SE-EGFP), the bacterium was detected in soil inoculated with >104 cfu/g and from most lettuce leaves cultivated in soils inoculated with >4.4 × 107 cfu/ g. As Salmonella was not detected in intact lettuce leaves or lettuce leaves with root injury cultivated in highly contaminated soils and after surface disinfection, the lettuce plants were not considered to internalize the bacterium. Overhead irrigation led to the contamination of one in 10 lettuce plants; however, all sets of three leaves of the plant were contaminated (>110 MPN/g). In an effort to prevent Salmonella contamination from soils, we investigated the effects of mulch on contamination levels during cultivation. Mulch effectively reduced Salmonella contamination levels of lettuce plants cultivated in highly contaminated soils.

UR - http://www.scopus.com/inward/record.url?scp=84912141416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84912141416&partnerID=8YFLogxK

U2 - 10.3136/fstr.20.961

DO - 10.3136/fstr.20.961

M3 - Article

AN - SCOPUS:84912141416

VL - 20

SP - 961

EP - 969

JO - Food Science and Technology Research

JF - Food Science and Technology Research

SN - 1344-6606

IS - 5

ER -