Investigation of sample behaviors inside on-chip electrophoresis microcapillary using confocal laser scanning microscopy

Shinichi Etoh, Toshihito Higashi, Tsuyoshi Fujimura, Reiji Hattori, Yukinori Kuroki

    Research output: Contribution to journalArticle

    Abstract

    We report the observation of sample behaviors using the confocal laser scanning microscopy (CLSM) in on-chip microcapillary. Sample loading by pinched valve injection is observed in a new cross injector shape, which has the structure added conventional cross injector to circle shape. In sample loading, because this structure causes a different electric field compared with that in conventional cross injector, high efficient sample plug injection was performed. It is important to investigate further the detailed sample profiles using the CLSM in sample loading for development of the on-chip microcapillary. We attempt the simulation of sample loading in the cross injector using the semiconductor device simulator MEDICI in order to investigate it in further detail. The sample movements in the channel turn along the Z-direction are observed using the CLSM. In order to miniaturize the microfluidic channel, it is necessarily needed to fold the channel, but then it is inevitable that sample dispersion occurs in the turn. We present sample flow profiles along the Z-direction in the turn using the CLSM and the influence on the electrophoretic separation. Also, We improve that fabrication of duct channel for exhaustion the vaporized xylene to outside the chip and the adhesion process.

    Original languageEnglish
    Pages (from-to)286-295
    Number of pages10
    JournalProceedings of SPIE - The International Society for Optical Engineering
    Volume5276
    DOIs
    Publication statusPublished - Jun 1 2004

    Fingerprint

    Laser Scanning
    Confocal
    electrophoresis
    Electrophoresis
    Microscopy
    Microscopic examination
    Chip
    chips
    microscopy
    Scanning
    scanning
    Lasers
    Injector
    lasers
    injectors
    Xylenes
    Xylene
    Semiconductor devices
    Microfluidics
    Ducts

    All Science Journal Classification (ASJC) codes

    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics
    • Computer Science Applications
    • Applied Mathematics
    • Electrical and Electronic Engineering

    Cite this

    Investigation of sample behaviors inside on-chip electrophoresis microcapillary using confocal laser scanning microscopy. / Etoh, Shinichi; Higashi, Toshihito; Fujimura, Tsuyoshi; Hattori, Reiji; Kuroki, Yukinori.

    In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 5276, 01.06.2004, p. 286-295.

    Research output: Contribution to journalArticle

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