Investigation of the effect of the c-kit inhibitor Glivec on isolated guinea-pig detrusor preparations

Yasue Kubota, Shunichi Kajioka, Suzanne M. Biers, Etsuko Yokota, Kenjiro Kohri, Alison F. Brading

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In order to assess the possible role of the c-kit positive cells in the bladder, the effects of c-kit tyrosine kinase inhibitor, Glivec®, on spontaneous excitation and ion channel activity in detrusor smooth muscles of the guinea-pig bladder were investigated using intracellular microelectrodes, isometric muscle tension recordings and patch clamp techniques. Glivec (10 μM) converted action potential bursts into continuous firing without affecting their shape but at 50 μM abolished spontaneous action potentials. It had little effect on inward and outward currents at <10 μM, but inhibited them at >50 μM. Glivec decreased the amplitude of spontaneous contractions dose dependently. These results suggest that c-kit positive cells may play a role in modulating spontaneous electrical and mechanical activities. Drugs inhibiting the c-kit receptor may provide a new approach for treating the overactive bladder.

Original languageEnglish
Pages (from-to)64-73
Number of pages10
JournalAutonomic Neuroscience: Basic and Clinical
Volume115
Issue number1-2
DOIs
Publication statusPublished - Sep 30 2004
Externally publishedYes

Fingerprint

Guinea Pigs
Action Potentials
Urinary Bladder
Proto-Oncogene Proteins c-kit
Overactive Urinary Bladder
Muscle Tonus
Microelectrodes
Patch-Clamp Techniques
Ion Channels
Protein-Tyrosine Kinases
Smooth Muscle
Pharmaceutical Preparations
Imatinib Mesylate

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems

Cite this

Investigation of the effect of the c-kit inhibitor Glivec on isolated guinea-pig detrusor preparations. / Kubota, Yasue; Kajioka, Shunichi; Biers, Suzanne M.; Yokota, Etsuko; Kohri, Kenjiro; Brading, Alison F.

In: Autonomic Neuroscience: Basic and Clinical, Vol. 115, No. 1-2, 30.09.2004, p. 64-73.

Research output: Contribution to journalArticle

Kubota, Yasue ; Kajioka, Shunichi ; Biers, Suzanne M. ; Yokota, Etsuko ; Kohri, Kenjiro ; Brading, Alison F. / Investigation of the effect of the c-kit inhibitor Glivec on isolated guinea-pig detrusor preparations. In: Autonomic Neuroscience: Basic and Clinical. 2004 ; Vol. 115, No. 1-2. pp. 64-73.
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