Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system

K. Miyamoto, T. Nakamura, Masayuki Tokita, T. Komai, M. Yonekawa, A. Kawamura, S. Adachi

Research output: Contribution to journalArticle

Abstract

Based on the concept that the granulocyte ratio (G/L) in cancer patients suggested the increase of G/L closely related to the tumor growth and the decrease to the depletion, we have been investigating the extracorporeal granulocyte adsorption system, depleting granulocyte mechanically, for the treatment of tumor bearing hosts. In order to clarify the therapeutic efficiency of this system, we have investigated the proteins adsorbed on the surfaces of granulocyte adsorption resin which was clinically applied. Extracted proteins obtained from the resin was analyzed with gel electrophoresis (SDS-Page) and ion exchange chromatography. These analyzed results revealed that the characteristic protein was only extracted with saline containing heparin, molecular weight of the protein was estimated over 300 kD. In Chromatogram, the peak of extracted proteins existed at the same position as that of Cellular Fibronectin-Heparin complex (cFN-Hep).

Original languageEnglish
Pages (from-to)690-694
Number of pages5
JournalJapanese Journal of Artificial Organs
Volume23
Issue number3
Publication statusPublished - Jan 1 1994

Fingerprint

Granulocytes
Proteins
Adsorption
Heparin
Neoplasms
Ion Exchange Chromatography
Fibronectins
Electrophoresis
Molecular Weight
Gels
Therapeutics
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

Miyamoto, K., Nakamura, T., Tokita, M., Komai, T., Yonekawa, M., Kawamura, A., & Adachi, S. (1994). Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system. Japanese Journal of Artificial Organs, 23(3), 690-694.

Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system. / Miyamoto, K.; Nakamura, T.; Tokita, Masayuki; Komai, T.; Yonekawa, M.; Kawamura, A.; Adachi, S.

In: Japanese Journal of Artificial Organs, Vol. 23, No. 3, 01.01.1994, p. 690-694.

Research output: Contribution to journalArticle

Miyamoto, K, Nakamura, T, Tokita, M, Komai, T, Yonekawa, M, Kawamura, A & Adachi, S 1994, 'Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system', Japanese Journal of Artificial Organs, vol. 23, no. 3, pp. 690-694.
Miyamoto, K. ; Nakamura, T. ; Tokita, Masayuki ; Komai, T. ; Yonekawa, M. ; Kawamura, A. ; Adachi, S. / Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system. In: Japanese Journal of Artificial Organs. 1994 ; Vol. 23, No. 3. pp. 690-694.
@article{b40f642075894a5bb2ff2cd1c74da760,
title = "Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system",
abstract = "Based on the concept that the granulocyte ratio (G/L) in cancer patients suggested the increase of G/L closely related to the tumor growth and the decrease to the depletion, we have been investigating the extracorporeal granulocyte adsorption system, depleting granulocyte mechanically, for the treatment of tumor bearing hosts. In order to clarify the therapeutic efficiency of this system, we have investigated the proteins adsorbed on the surfaces of granulocyte adsorption resin which was clinically applied. Extracted proteins obtained from the resin was analyzed with gel electrophoresis (SDS-Page) and ion exchange chromatography. These analyzed results revealed that the characteristic protein was only extracted with saline containing heparin, molecular weight of the protein was estimated over 300 kD. In Chromatogram, the peak of extracted proteins existed at the same position as that of Cellular Fibronectin-Heparin complex (cFN-Hep).",
author = "K. Miyamoto and T. Nakamura and Masayuki Tokita and T. Komai and M. Yonekawa and A. Kawamura and S. Adachi",
year = "1994",
month = "1",
day = "1",
language = "English",
volume = "23",
pages = "690--694",
journal = "Japanese Journal of Artificial Organs",
issn = "0300-0818",
publisher = "Japanese Society for Artificial Organs and Tissues",
number = "3",

}

TY - JOUR

T1 - Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system

AU - Miyamoto, K.

AU - Nakamura, T.

AU - Tokita, Masayuki

AU - Komai, T.

AU - Yonekawa, M.

AU - Kawamura, A.

AU - Adachi, S.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Based on the concept that the granulocyte ratio (G/L) in cancer patients suggested the increase of G/L closely related to the tumor growth and the decrease to the depletion, we have been investigating the extracorporeal granulocyte adsorption system, depleting granulocyte mechanically, for the treatment of tumor bearing hosts. In order to clarify the therapeutic efficiency of this system, we have investigated the proteins adsorbed on the surfaces of granulocyte adsorption resin which was clinically applied. Extracted proteins obtained from the resin was analyzed with gel electrophoresis (SDS-Page) and ion exchange chromatography. These analyzed results revealed that the characteristic protein was only extracted with saline containing heparin, molecular weight of the protein was estimated over 300 kD. In Chromatogram, the peak of extracted proteins existed at the same position as that of Cellular Fibronectin-Heparin complex (cFN-Hep).

AB - Based on the concept that the granulocyte ratio (G/L) in cancer patients suggested the increase of G/L closely related to the tumor growth and the decrease to the depletion, we have been investigating the extracorporeal granulocyte adsorption system, depleting granulocyte mechanically, for the treatment of tumor bearing hosts. In order to clarify the therapeutic efficiency of this system, we have investigated the proteins adsorbed on the surfaces of granulocyte adsorption resin which was clinically applied. Extracted proteins obtained from the resin was analyzed with gel electrophoresis (SDS-Page) and ion exchange chromatography. These analyzed results revealed that the characteristic protein was only extracted with saline containing heparin, molecular weight of the protein was estimated over 300 kD. In Chromatogram, the peak of extracted proteins existed at the same position as that of Cellular Fibronectin-Heparin complex (cFN-Hep).

UR - http://www.scopus.com/inward/record.url?scp=0028365167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028365167&partnerID=8YFLogxK

M3 - Article

VL - 23

SP - 690

EP - 694

JO - Japanese Journal of Artificial Organs

JF - Japanese Journal of Artificial Organs

SN - 0300-0818

IS - 3

ER -