Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system

K. Miyamoto, T. Nakamura, Masayuki Tokita, T. Komai, M. Yonekawa, A. Kawamura, S. Adachi

Research output: Contribution to journalArticle

Abstract

Based on the concept that the granulocyte ratio (G/L) in cancer patients suggested the increase of G/L closely related to the tumor growth and the decrease to the depletion, we have been investigating the extracorporeal granulocyte adsorption system, depleting granulocyte mechanically, for the treatment of tumor bearing hosts. In order to clarify the therapeutic efficiency of this system, we have investigated the proteins adsorbed on the surfaces of granulocyte adsorption resin which was clinically applied. Extracted proteins obtained from the resin was analyzed with gel electrophoresis (SDS-Page) and ion exchange chromatography. These analyzed results revealed that the characteristic protein was only extracted with saline containing heparin, molecular weight of the protein was estimated over 300 kD. In Chromatogram, the peak of extracted proteins existed at the same position as that of Cellular Fibronectin-Heparin complex (cFN-Hep).

Original languageEnglish
Pages (from-to)690-694
Number of pages5
JournalJapanese Journal of Artificial Organs
Volume23
Issue number3
Publication statusPublished - Jan 1 1994

All Science Journal Classification (ASJC) codes

  • Biophysics

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    Miyamoto, K., Nakamura, T., Tokita, M., Komai, T., Yonekawa, M., Kawamura, A., & Adachi, S. (1994). Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system. Japanese Journal of Artificial Organs, 23(3), 690-694.