Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system

Based on the concept that the granulocyte ratio (G/L) in cancer patients suggested the increase of G/L closely related to the tumor growth and the decrease to the depletion, we have been investigating the extracorporeal granulocyte adsorption system, depleting granulocyte mechanically, for the treatment of tumor bearing hosts. In order to clarify the therapeutic efficiency of this system, we have investigated the proteins adsorbed on the surfaces of granulocyte adsorption resin which was clinically applied. Extracted proteins obtained from the resin was analyzed with gel electrophoresis (SDS-Page) and ion exchange chromatography. These analyzed results revealed that the characteristic protein was only extracted with saline containing heparin, molecular weight of the protein was estimated over 300 kD. In Chromatogram, the peak of extracted proteins existed at the same position as that of Cellular Fibronectin-Heparin complex (cFN-Hep).