TY - JOUR
T1 - Investigation of the role of CD1d-restricted invariant NKT cells in experimental choroidal neovascularization
AU - Hijioka, Kuniaki
AU - Sonoda, Koh Hei
AU - Tsutsumi-Miyahara, Chikako
AU - Fujimoto, Takeshi
AU - Oshima, Yuji
AU - Taniguchi, Masaru
AU - Ishibashi, Tatsuro
N1 - Funding Information:
Grants: This work was supported by grants from the Ministry of Education, Science, Sports and Culture, Japan (B1 No. 18390469: K.-H. Sonoda).
PY - 2008/9/12
Y1 - 2008/9/12
N2 - Choroidal neovascularization (CNV) is directly related to visual loss in age-related macular degeneration and other macular disorders. We have investigated the role of CD1d-restricted invariant natural killer T (NKT) cells in laser-induced experimental CNV. Quantitative real-time PCR detected increased expression of NKT cell-related genes (Vα14 and CXCL16) in whole eyes undergoing CNV, indicating local accumulation of NKT cells. We found a significant reduction of CNV and lower concentrations of vascular endothelial growth factor (VEGF) in ocular fluid in two different NKT cell-deficient mice, CD1d knockout (KO) and Jα18 KO mice. We also established in vitro co-cultures of retinal pigment epithelial cells and splenic NKT cells, and confirmed NKT cells could produce VEGF in the dish. Moreover, inoculating α-galactosylceramide, the ligand for NKT cells, into the vitreous cavity of C57BL/6 mice promoted CNV. We concluded that NKT cells play an important role in CNV as an inducer of VEGF.
AB - Choroidal neovascularization (CNV) is directly related to visual loss in age-related macular degeneration and other macular disorders. We have investigated the role of CD1d-restricted invariant natural killer T (NKT) cells in laser-induced experimental CNV. Quantitative real-time PCR detected increased expression of NKT cell-related genes (Vα14 and CXCL16) in whole eyes undergoing CNV, indicating local accumulation of NKT cells. We found a significant reduction of CNV and lower concentrations of vascular endothelial growth factor (VEGF) in ocular fluid in two different NKT cell-deficient mice, CD1d knockout (KO) and Jα18 KO mice. We also established in vitro co-cultures of retinal pigment epithelial cells and splenic NKT cells, and confirmed NKT cells could produce VEGF in the dish. Moreover, inoculating α-galactosylceramide, the ligand for NKT cells, into the vitreous cavity of C57BL/6 mice promoted CNV. We concluded that NKT cells play an important role in CNV as an inducer of VEGF.
UR - http://www.scopus.com/inward/record.url?scp=47749090863&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=47749090863&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.06.080
DO - 10.1016/j.bbrc.2008.06.080
M3 - Article
C2 - 18606153
AN - SCOPUS:47749090863
SN - 0006-291X
VL - 374
SP - 38
EP - 43
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -