TY - JOUR
T1 - Involvement of 5-hydroxytryptamine neuronal system in Δ9-tetrahydrocannabinol-induced impairment of spatial memory
AU - Egashira, Nobuaki
AU - Mishima, Kenichi
AU - Katsurabayashi, Shutaro
AU - Yoshitake, Tomohiro
AU - Matsumoto, Yoshiaki
AU - Ishida, Junichi
AU - Yamaguchi, Masatoshi
AU - Iwasaki, Katsunori
AU - Fujiwara, Michihiro
N1 - Funding Information:
The authors are grateful to Professor Y. Shoyama, Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, for kindly supplying natural Δ 9 -tetrahydrocannabinol. The financial support of the Grant-in-Aid for Scientific Research (No. 12771472) from the Ministry of Education, Science and Culture of Japan and the Research Grant (10A-3 and 13A-3) for Nervous and Mental Disorders from the Ministry of Health and Welfare is greatly acknowledged. We are grateful to Mr. T. Uchida, Mr. H. Aoki, Miss K. Nakatsugawa, Mr. K. Shintaku, Miss A. Yano, Miss R. Yukawa, Miss A. Yano, Miss K. Kunichika, Miss H. Hara, Miss Y. Shigematsu and Miss Y. Masaki for their skillful technical assistance.
PY - 2002/6/12
Y1 - 2002/6/12
N2 - The present study investigated the involvement of the serotonin (5-hydroxytryptamine, 5-HT) neuronal system in the Δ9-tetrahydrocannabinol-induced impairment of spatial memory in the eight-arm radial maze in rats. Δ9-Tetrahydrocannabinol (6 mg/kg, i.p.), which impairs spatial memory, significantly increased the 5-HT content in the ventral hippocampus. A microdialysis study showed that Δ9-tetrahydrocannabinol (6 mg/kg, i.p.) decreased 5-HT release in the ventral hippocampus. The 5-HT precursor, 5-hydroxy-L-tryptophan (5-HTP; 50 mg/kg, i.p.), the 5-HT re-uptake inhibitor, clomipramine (0.01 and 0.1 mg/kg, i.p.), the 5-HT receptor agonist, 5-methoxy-N, N-dimethyltryptamine (5-MeODMT; 0.01 and 0.03 mg/kg, i.p.), and the 5-HT2 receptor agonist, 1-(2,5-dimethoxy 4-iodophenyl)-2-amino propane (DOI; 10 μg/kg, i.p.), significantly attenuated the Δ9-tetrahydrocannabinol-induced impairment of spatial memory. These results suggest that the 5-HT neuronal system may be involved in the Δ9-tetrahydrocannabinol-induced impairment of spatial memory.
AB - The present study investigated the involvement of the serotonin (5-hydroxytryptamine, 5-HT) neuronal system in the Δ9-tetrahydrocannabinol-induced impairment of spatial memory in the eight-arm radial maze in rats. Δ9-Tetrahydrocannabinol (6 mg/kg, i.p.), which impairs spatial memory, significantly increased the 5-HT content in the ventral hippocampus. A microdialysis study showed that Δ9-tetrahydrocannabinol (6 mg/kg, i.p.) decreased 5-HT release in the ventral hippocampus. The 5-HT precursor, 5-hydroxy-L-tryptophan (5-HTP; 50 mg/kg, i.p.), the 5-HT re-uptake inhibitor, clomipramine (0.01 and 0.1 mg/kg, i.p.), the 5-HT receptor agonist, 5-methoxy-N, N-dimethyltryptamine (5-MeODMT; 0.01 and 0.03 mg/kg, i.p.), and the 5-HT2 receptor agonist, 1-(2,5-dimethoxy 4-iodophenyl)-2-amino propane (DOI; 10 μg/kg, i.p.), significantly attenuated the Δ9-tetrahydrocannabinol-induced impairment of spatial memory. These results suggest that the 5-HT neuronal system may be involved in the Δ9-tetrahydrocannabinol-induced impairment of spatial memory.
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U2 - 10.1016/S0014-2999(02)01755-7
DO - 10.1016/S0014-2999(02)01755-7
M3 - Article
C2 - 12079687
AN - SCOPUS:0037066909
SN - 0014-2999
VL - 445
SP - 221
EP - 229
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 3
ER -