Involvement of AfsA in A-factor biosynthesis as a key enzyme

Noriko Ando, Nobuaki Matsumori, Shohei Sakuda, Teruhiko Beppu, Sueharu Horinouchi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The afsA gene of Streptomyces griseus has been postulated to encode a key enzyme for A-factor biosynthesis from primary metabolites commonly present in Streptomyces strains. Escherichia coli cells harboring afsA under the control of the T7 promoter specified distinct A-factor activity in the culture broth, as determined by induction of streptomycin production and aerial mycelium and spore formation in an A-factor-deficient S. griseus mutant strain. Production of the substance(s) having A-factor activity was inhibited by cerulenin, an inhibitor of fatty acid biosynthesis. These observations suggest that afsA encodes a key enzyme in the A-factor biosynthetic pathway in which a β-keto acid derived from fatty acid biosynthesis and a glycerol derivative serve as precursors.

Original languageEnglish
Pages (from-to)847-852
Number of pages6
JournalJournal of Antibiotics
Volume50
Issue number10
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

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