Involvement of blood-group-B-active trisaccharides in Ca2+-dependent cell-cell adhesion in the Xenopus blastula

Kazuko H. Nomura, Ryuji Kobayashi, Yoshio Hirabayashi, Megumi Fujisue-Sakai, Souhei Mizuguchi, Kazuya Nomura

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Despite their wide distribution in various organisms, no physiological roles have been proposed for the human blood-group-ABO (ABH)-active trisaccharides. Here we show that monoclonal antibodies against human blood-group-B-active trisaccharides (B-substance) completely block the Ca2+-dependent cell-cell adhesion system of frog (Xenopus laevis) embryonic cells. Synthetic B-substance or B-active glycopeptides also disrupt the Ca2+-dependent cell-cell adhesion. These results suggest that blood-group-B-active substances play a role in cell-cell adhesion. Blood-group-B-active substances were found as glycoproteins and as glycosphingolipids. In order to identify B-active glycoproteins active in cell-cell adhesion, we purified B-active membrane glycoproteins by two-dimensional electrophoresis and found that they are 45- to 58-kDa proteins with pI(s) ranging from 4.0 to 5.3. They are glycosylphosphatidyl inositol (GPI) anchored. Amino acid sequence analysis showed that the purified B-active GPI-anchored proteins are homologues of soluble Xenopus cortical granule lectins (CGL). The results suggest that the B-active membrane glycoproteins are GPI-anchored forms of the lectin and are directly involved in frog Ca2+-dependent cell-cell adhesion.

Original languageEnglish
Pages (from-to)9-18
Number of pages10
JournalDevelopment Genes and Evolution
Volume208
Issue number1
DOIs
Publication statusPublished - 1998

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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