Involvement of blood-group-B-active trisaccharides in Ca2+-dependent cell-cell adhesion in the Xenopus blastula

Kazuko H. Nomura, Ryuji Kobayashi, Yoshio Hirabayashi, Megumi Fujisue-Sakai, Souhei Mizuguchi, Kazuya Nomura

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Despite their wide distribution in various organisms, no physiological roles have been proposed for the human blood-group-ABO (ABH)-active trisaccharides. Here we show that monoclonal antibodies against human blood-group-B-active trisaccharides (B-substance) completely block the Ca2+-dependent cell-cell adhesion system of frog (Xenopus laevis) embryonic cells. Synthetic B-substance or B-active glycopeptides also disrupt the Ca2+-dependent cell-cell adhesion. These results suggest that blood-group-B-active substances play a role in cell-cell adhesion. Blood-group-B-active substances were found as glycoproteins and as glycosphingolipids. In order to identify B-active glycoproteins active in cell-cell adhesion, we purified B-active membrane glycoproteins by two-dimensional electrophoresis and found that they are 45- to 58-kDa proteins with pI(s) ranging from 4.0 to 5.3. They are glycosylphosphatidyl inositol (GPI) anchored. Amino acid sequence analysis showed that the purified B-active GPI-anchored proteins are homologues of soluble Xenopus cortical granule lectins (CGL). The results suggest that the B-active membrane glycoproteins are GPI-anchored forms of the lectin and are directly involved in frog Ca2+-dependent cell-cell adhesion.

Original languageEnglish
Pages (from-to)9-18
Number of pages10
JournalDevelopment Genes and Evolution
Volume208
Issue number1
DOIs
Publication statusPublished - Apr 27 1998

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Blastula
Xenopus
Cell Adhesion
Inositol
Membrane Glycoproteins
Blood Group Antigens
Lectins
Anura
Glycoproteins
ABO Blood-Group System
Trisaccharides
Glycosphingolipids
Glycopeptides
Protein Sequence Analysis
Xenopus laevis
Electrophoresis
blood group B trisaccharide
Proteins
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Nomura, K. H., Kobayashi, R., Hirabayashi, Y., Fujisue-Sakai, M., Mizuguchi, S., & Nomura, K. (1998). Involvement of blood-group-B-active trisaccharides in Ca2+-dependent cell-cell adhesion in the Xenopus blastula. Development Genes and Evolution, 208(1), 9-18. https://doi.org/10.1007/s004270050148

Involvement of blood-group-B-active trisaccharides in Ca2+-dependent cell-cell adhesion in the Xenopus blastula. / Nomura, Kazuko H.; Kobayashi, Ryuji; Hirabayashi, Yoshio; Fujisue-Sakai, Megumi; Mizuguchi, Souhei; Nomura, Kazuya.

In: Development Genes and Evolution, Vol. 208, No. 1, 27.04.1998, p. 9-18.

Research output: Contribution to journalArticle

Nomura, KH, Kobayashi, R, Hirabayashi, Y, Fujisue-Sakai, M, Mizuguchi, S & Nomura, K 1998, 'Involvement of blood-group-B-active trisaccharides in Ca2+-dependent cell-cell adhesion in the Xenopus blastula', Development Genes and Evolution, vol. 208, no. 1, pp. 9-18. https://doi.org/10.1007/s004270050148
Nomura, Kazuko H. ; Kobayashi, Ryuji ; Hirabayashi, Yoshio ; Fujisue-Sakai, Megumi ; Mizuguchi, Souhei ; Nomura, Kazuya. / Involvement of blood-group-B-active trisaccharides in Ca2+-dependent cell-cell adhesion in the Xenopus blastula. In: Development Genes and Evolution. 1998 ; Vol. 208, No. 1. pp. 9-18.
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