Involvement of Ca2+ in globular adiponectin-induced reactive oxygen species

Sumio Akifusa, Noriaki Kamio, Yoshihiro Shimazaki, Noboru Yamaguchi, Yoshihisa Yamashita

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. We investigated the role of Ca2+ in gAd-induced ROS and NO generation. Pretreatment with BAPTA-AM, a selective chelator of intracellular Ca2+ ([Ca2+]i), partially reduced gAd-induced generation of ROS and NO in gAd-treated RAW 264 cells. The lowest [Ca2+]i occurred 30 min after gAd treatment, after which [Ca2+]i increased continually and exceeded the initial level. The mitochondrial Ca2+ ([Ca2+]m) detected by Rhod-2 fluorescence started to increase at 6 h after gAd treatment. Pretreatment with a NAD(P)H oxidase inhibitor, diphenyleneiodonium, prevented the reduction of [Ca2+]i in the early phase after gAd treatment. Calcium depletion by BAPTA-AM had no effect on the gAd-induced [Ca2+]m oscillation. The administration of a specific calmodulin inhibitor, calmidazolium, significantly suppressed gAd-induced ROS and NO generation and NOS activity.

Original languageEnglish
Pages (from-to)649-653
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume381
Issue number4
DOIs
Publication statusPublished - Apr 17 2009

Fingerprint

Adiponectin
Reactive Oxygen Species
Nitric Oxide
calmidazolium
Macrophages
NADPH Oxidase
Calmodulin
Chelating Agents
Fluorescence
Cells
Calcium
Cell Line

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Involvement of Ca2+ in globular adiponectin-induced reactive oxygen species. / Akifusa, Sumio; Kamio, Noriaki; Shimazaki, Yoshihiro; Yamaguchi, Noboru; Yamashita, Yoshihisa.

In: Biochemical and Biophysical Research Communications, Vol. 381, No. 4, 17.04.2009, p. 649-653.

Research output: Contribution to journalArticle

Akifusa, Sumio ; Kamio, Noriaki ; Shimazaki, Yoshihiro ; Yamaguchi, Noboru ; Yamashita, Yoshihisa. / Involvement of Ca2+ in globular adiponectin-induced reactive oxygen species. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 381, No. 4. pp. 649-653.
@article{16b6f032524c4ebdba3d2ff5f7a1b8b6,
title = "Involvement of Ca2+ in globular adiponectin-induced reactive oxygen species",
abstract = "Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. We investigated the role of Ca2+ in gAd-induced ROS and NO generation. Pretreatment with BAPTA-AM, a selective chelator of intracellular Ca2+ ([Ca2+]i), partially reduced gAd-induced generation of ROS and NO in gAd-treated RAW 264 cells. The lowest [Ca2+]i occurred 30 min after gAd treatment, after which [Ca2+]i increased continually and exceeded the initial level. The mitochondrial Ca2+ ([Ca2+]m) detected by Rhod-2 fluorescence started to increase at 6 h after gAd treatment. Pretreatment with a NAD(P)H oxidase inhibitor, diphenyleneiodonium, prevented the reduction of [Ca2+]i in the early phase after gAd treatment. Calcium depletion by BAPTA-AM had no effect on the gAd-induced [Ca2+]m oscillation. The administration of a specific calmodulin inhibitor, calmidazolium, significantly suppressed gAd-induced ROS and NO generation and NOS activity.",
author = "Sumio Akifusa and Noriaki Kamio and Yoshihiro Shimazaki and Noboru Yamaguchi and Yoshihisa Yamashita",
year = "2009",
month = "4",
day = "17",
doi = "10.1016/j.bbrc.2009.02.115",
language = "English",
volume = "381",
pages = "649--653",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Involvement of Ca2+ in globular adiponectin-induced reactive oxygen species

AU - Akifusa, Sumio

AU - Kamio, Noriaki

AU - Shimazaki, Yoshihiro

AU - Yamaguchi, Noboru

AU - Yamashita, Yoshihisa

PY - 2009/4/17

Y1 - 2009/4/17

N2 - Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. We investigated the role of Ca2+ in gAd-induced ROS and NO generation. Pretreatment with BAPTA-AM, a selective chelator of intracellular Ca2+ ([Ca2+]i), partially reduced gAd-induced generation of ROS and NO in gAd-treated RAW 264 cells. The lowest [Ca2+]i occurred 30 min after gAd treatment, after which [Ca2+]i increased continually and exceeded the initial level. The mitochondrial Ca2+ ([Ca2+]m) detected by Rhod-2 fluorescence started to increase at 6 h after gAd treatment. Pretreatment with a NAD(P)H oxidase inhibitor, diphenyleneiodonium, prevented the reduction of [Ca2+]i in the early phase after gAd treatment. Calcium depletion by BAPTA-AM had no effect on the gAd-induced [Ca2+]m oscillation. The administration of a specific calmodulin inhibitor, calmidazolium, significantly suppressed gAd-induced ROS and NO generation and NOS activity.

AB - Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. We investigated the role of Ca2+ in gAd-induced ROS and NO generation. Pretreatment with BAPTA-AM, a selective chelator of intracellular Ca2+ ([Ca2+]i), partially reduced gAd-induced generation of ROS and NO in gAd-treated RAW 264 cells. The lowest [Ca2+]i occurred 30 min after gAd treatment, after which [Ca2+]i increased continually and exceeded the initial level. The mitochondrial Ca2+ ([Ca2+]m) detected by Rhod-2 fluorescence started to increase at 6 h after gAd treatment. Pretreatment with a NAD(P)H oxidase inhibitor, diphenyleneiodonium, prevented the reduction of [Ca2+]i in the early phase after gAd treatment. Calcium depletion by BAPTA-AM had no effect on the gAd-induced [Ca2+]m oscillation. The administration of a specific calmodulin inhibitor, calmidazolium, significantly suppressed gAd-induced ROS and NO generation and NOS activity.

UR - http://www.scopus.com/inward/record.url?scp=62649096153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62649096153&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.02.115

DO - 10.1016/j.bbrc.2009.02.115

M3 - Article

VL - 381

SP - 649

EP - 653

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -