TY - JOUR
T1 - Involvement of caveolin-2 in caveolar biogenesis in MDCK cells
AU - Lahtinen, Ulla
AU - Honsho, Masanori
AU - Parton, Robert G.
AU - Simons, Kai
AU - Verkade, Paul
N1 - Funding Information:
We thank Kim Ekroos and Beate Michel for cell culture, Patrick Keller for advice with the adenovirus system and Kurt Anderson for immunofluorescence quantitation. Masanori Honsho was partially supported by the Uehara memorial foundation.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2003/3/13
Y1 - 2003/3/13
N2 - Caveolins have been identified as key components of caveolae, specialized cholesterol-enriched raft domains visible as small flask-shaped invaginations of the plasma membrane. In polarized MDCK cells caveolin-1 and -2 are found together on basolateral caveolae whereas the apical membrane, where only caveolin-1 is present, lacks caveolae. Expression of a caveolin mutant prevented the formation of the large caveolin-1/-2 hetero-oligomeric complexes, and led to intracellular retention of caveolin-2 and disappearance of caveolae from the basolateral membrane. Correspondingly, in MDCK cells over-expressing caveolin-2 the basolateral membrane exhibited an increased number of caveolae. These results indicate the involvement of caveolin-2 in caveolar biogenesis.
AB - Caveolins have been identified as key components of caveolae, specialized cholesterol-enriched raft domains visible as small flask-shaped invaginations of the plasma membrane. In polarized MDCK cells caveolin-1 and -2 are found together on basolateral caveolae whereas the apical membrane, where only caveolin-1 is present, lacks caveolae. Expression of a caveolin mutant prevented the formation of the large caveolin-1/-2 hetero-oligomeric complexes, and led to intracellular retention of caveolin-2 and disappearance of caveolae from the basolateral membrane. Correspondingly, in MDCK cells over-expressing caveolin-2 the basolateral membrane exhibited an increased number of caveolae. These results indicate the involvement of caveolin-2 in caveolar biogenesis.
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U2 - 10.1016/S0014-5793(03)00135-2
DO - 10.1016/S0014-5793(03)00135-2
M3 - Article
C2 - 12633858
AN - SCOPUS:0037434839
SN - 0014-5793
VL - 538
SP - 85
EP - 88
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -