Involvement of CD4+,CD57+ T cells in the disease activity of rheumatoid arthritis

Takeshi Maeda, Hisakata Yamada, Ryuji Nagamine, Toshihide Shuto, Yasuharu Nakashima, Go Hirata, Yukihide Iwamoto

Research output: Contribution to journalArticle

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Abstract

Objective. To evaluate the relationship between the frequency of peripheral CD57+ T cells and the physical status of rheumatoid arthritis (RA) patients, and to perform cytokine analysis of these CD57+ T cells. Methods. Four-color fluorescence-activated cell sorter analysis was performed to detect both cell surface antigens and intracellular cytokines in peripheral blood leukocytes, using monoclonal antibodies against CD3, CD4, CD8, CD57, interferon-γ (IFN-γ), and interleukin-4 (IL-4). RA patients were clinically evaluated with a modified Health Assessment Questionnaire (M-HAQ), joint score, face scale, and visual analog scale (VAS) assessing pain and disease activity. Results. There was a significant correlation between the frequency of CD4+,CD57+ T cells and erythrocyte sedimentation rate (ESR), whereas a correlation was not found between the frequency of CD8+,CD57+ T cells and ESR. The frequency of CD4+,CD57+ T cells also showed a significant correlation with the mHAQ score, VAS, and face scale. Again, there was no significant correlation between the above-mentioned clinical scores and the frequency of CD8+,CD57+ T cells. Flow cytometric analysis of intracellular cytokines revealed that 14.5% of the CD57+ T cells produced IFNγ, whereas only 2.8% of the CD57+ T cells produced IL-4 in RA patients. Conclusion. Evidence showing that the frequency of CD4+,CD57+ T cells among CD3+ cells of RA patients had a significant correlation not only with ESR but also with the physical status of the patients, and that a large proportion of the CD4+,CD57+ T cells had the capacity to produce IFNγ, strongly suggests that these CD4+,CD57+ T cells are involved in the immunopathogenesis of RA.

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalArthritis and rheumatism
Volume46
Issue number2
DOIs
Publication statusPublished - Feb 20 2002

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Rheumatoid Arthritis
T-Lymphocytes
Blood Sedimentation
Cytokines
Interleukin-4
Pain Measurement
Surface Antigens
Visual Analog Scale
Interferons
Leukocytes
Color
Joints
Fluorescence
Monoclonal Antibodies
Health

All Science Journal Classification (ASJC) codes

  • Immunology
  • Rheumatology

Cite this

Maeda, T., Yamada, H., Nagamine, R., Shuto, T., Nakashima, Y., Hirata, G., & Iwamoto, Y. (2002). Involvement of CD4+,CD57+ T cells in the disease activity of rheumatoid arthritis. Arthritis and rheumatism, 46(2), 379-384. https://doi.org/10.1002/art.10133

Involvement of CD4+,CD57+ T cells in the disease activity of rheumatoid arthritis. / Maeda, Takeshi; Yamada, Hisakata; Nagamine, Ryuji; Shuto, Toshihide; Nakashima, Yasuharu; Hirata, Go; Iwamoto, Yukihide.

In: Arthritis and rheumatism, Vol. 46, No. 2, 20.02.2002, p. 379-384.

Research output: Contribution to journalArticle

Maeda, T, Yamada, H, Nagamine, R, Shuto, T, Nakashima, Y, Hirata, G & Iwamoto, Y 2002, 'Involvement of CD4+,CD57+ T cells in the disease activity of rheumatoid arthritis', Arthritis and rheumatism, vol. 46, no. 2, pp. 379-384. https://doi.org/10.1002/art.10133
Maeda, Takeshi ; Yamada, Hisakata ; Nagamine, Ryuji ; Shuto, Toshihide ; Nakashima, Yasuharu ; Hirata, Go ; Iwamoto, Yukihide. / Involvement of CD4+,CD57+ T cells in the disease activity of rheumatoid arthritis. In: Arthritis and rheumatism. 2002 ; Vol. 46, No. 2. pp. 379-384.
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