TY - JOUR
T1 - Involvement of clusterin in 15-deoxy-Δ12,14-prostaglandin J2-induced vascular smooth muscle cell differentiation
AU - Miwa, Yoshikazu
AU - Takahashi-Yanaga, Fumi
AU - Morimoto, Sachio
AU - Sasaguri, Toshiyuki
N1 - Funding Information:
This study was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/6/18
Y1 - 2004/6/18
N2 - To establish an in vitro model of vascular smooth muscle cell (VSMC) differentiation, we examined the effect of 15-deoxy-Δ12,14- prostaglandin J2 (15d-PGJ2) on the expression of VSMC differentiation markers. After the addition of 15d-PGJ2 to confluent human umbilical artery smooth muscle cells synchronized in the G0 phase, cells showed a "hill and valley" appearance and thereafter aggregated and formed macroscopic nodules. Cells forming nodules expressed high levels of SM2, the most specific VSMC differentiation marker, comparable to medial VSMCs in vivo. 15d-PGJ2 significantly increased the mRNA and protein expression levels of clusterin, a secreted glycoprotein reported to induce nodule formation and differentiation of VSMCs. Moreover, addition of an anti-clusterin antibody completely inhibited the nodule formation induced by 15d-PGJ2 and induced apoptosis. Our results suggested that clusterin is involved in 15d-PGJ2-induced nodule formation and cell differentiation in VSMCs.
AB - To establish an in vitro model of vascular smooth muscle cell (VSMC) differentiation, we examined the effect of 15-deoxy-Δ12,14- prostaglandin J2 (15d-PGJ2) on the expression of VSMC differentiation markers. After the addition of 15d-PGJ2 to confluent human umbilical artery smooth muscle cells synchronized in the G0 phase, cells showed a "hill and valley" appearance and thereafter aggregated and formed macroscopic nodules. Cells forming nodules expressed high levels of SM2, the most specific VSMC differentiation marker, comparable to medial VSMCs in vivo. 15d-PGJ2 significantly increased the mRNA and protein expression levels of clusterin, a secreted glycoprotein reported to induce nodule formation and differentiation of VSMCs. Moreover, addition of an anti-clusterin antibody completely inhibited the nodule formation induced by 15d-PGJ2 and induced apoptosis. Our results suggested that clusterin is involved in 15d-PGJ2-induced nodule formation and cell differentiation in VSMCs.
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U2 - 10.1016/j.bbrc.2004.04.163
DO - 10.1016/j.bbrc.2004.04.163
M3 - Article
C2 - 15158456
AN - SCOPUS:2442718691
VL - 319
SP - 163
EP - 168
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -