Involvement of heparan sulfate proteoglycan in sensing hypotonic stress in bovine aortic endothelial cells

Masahiro Oike, Michi Watanabe, Kimura Chiwaka

Research output: Contribution to journalArticle

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Abstract

Hypotonic stress (HTS) induces various responses in vascular endothelium, but the molecules involved in sensing HTS are not known. To investigate a possible role of heparan sulfate proteoglycan (HSPG) in sensing HTS, we compared the responses of control bovine aortic endothelial cells (BAECs) with those of cells treated with heparinase III, which exclusively degrades HSPG. Tyrosine phosphorylation of 125 kDa FAK induced by HTS (- 30%) in control cells was abolished in heparinase III-treated BAECs. The amplitude of the volume-regulated anion channel (VRAC) current, whose activation is regulated by tyrosine kinase, was significantly reduced by the treatment with heparinase III. Also, HTS-induced ATP release through the VRAC pore and the concomitant Ca2+ transients were significantly reduced in the heparinase III-treated BAECs. In contrast, exogenously applied ATP evoked similar Ca2+ transients in both control and heparinase III-treated BAECs. The transient formation of actin stress fibers induced by HTS in control cells was absent in heparinase III-treated BAECs. Lysophosphatidic acid (LPA) also induced FAK phosphorylation, actin reorganization and ATP release in control BAECs, but heparinase III did not affect these LPA-induced responses. We conclude from these observations that HSPG is one of the sensory molecules of hypotonic cell swelling in BAECs.

Original languageEnglish
Pages (from-to)1148-1155
Number of pages8
JournalBiochimica et Biophysica Acta - General Subjects
Volume1780
Issue number10
DOIs
Publication statusPublished - Oct 1 2008

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heparitinsulfate lyase
Heparan Sulfate Proteoglycans
Endothelial cells
Osmotic Pressure
Endothelial Cells
Phosphorylation
Anions
Adenosine Triphosphate
Stress Fibers
Molecules
Vascular Endothelium
Protein-Tyrosine Kinases
Tyrosine
Swelling
Actins
Chemical activation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Involvement of heparan sulfate proteoglycan in sensing hypotonic stress in bovine aortic endothelial cells. / Oike, Masahiro; Watanabe, Michi; Chiwaka, Kimura.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1780, No. 10, 01.10.2008, p. 1148-1155.

Research output: Contribution to journalArticle

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