Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells

Makiko Yamashita, Yoshinori Katakura, Yoshihiro Aiba, Shin Ei Matsumoto, Kazuko Morihara, Kiichiro Teruya, Akira Ichikawa, Sanetaka Shirahata

Research output: Contribution to journalArticle

Abstract

Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with l-leucyl-l-leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4+ Th cells and CD11c+ DC, resulting in the suppression of antibody production by in vitro immunized PBMC.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalCytotechnology
Volume55
Issue number2-3
DOIs
Publication statusPublished - Dec 2007

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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