Abstract
Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with l-leucyl-l-leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4+ Th cells and CD11c+ DC, resulting in the suppression of antibody production by in vitro immunized PBMC.
| Original language | English |
|---|---|
| Pages (from-to) | 71-77 |
| Number of pages | 7 |
| Journal | Cytotechnology |
| Volume | 55 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - Dec 1 2007 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Clinical Biochemistry
- Cell Biology
Cite this
Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells. / Yamashita, Makiko; Katakura, Yoshinori; Aiba, Yoshihiro; Matsumoto, Shin Ei; Morihara, Kazuko; Teruya, Kiichiro; Ichikawa, Akira; Shirahata, Sanetaka.
In: Cytotechnology, Vol. 55, No. 2-3, 01.12.2007, p. 71-77.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells
AU - Yamashita, Makiko
AU - Katakura, Yoshinori
AU - Aiba, Yoshihiro
AU - Matsumoto, Shin Ei
AU - Morihara, Kazuko
AU - Teruya, Kiichiro
AU - Ichikawa, Akira
AU - Shirahata, Sanetaka
PY - 2007/12/1
Y1 - 2007/12/1
N2 - Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with l-leucyl-l-leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4+ Th cells and CD11c+ DC, resulting in the suppression of antibody production by in vitro immunized PBMC.
AB - Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with l-leucyl-l-leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4+ Th cells and CD11c+ DC, resulting in the suppression of antibody production by in vitro immunized PBMC.
UR - http://www.scopus.com/inward/record.url?scp=36749079207&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36749079207&partnerID=8YFLogxK
U2 - 10.1007/s10616-007-9088-x
DO - 10.1007/s10616-007-9088-x
M3 - Article
C2 - 19002996
AN - SCOPUS:36749079207
VL - 55
SP - 71
EP - 77
JO - Cytotechnology
JF - Cytotechnology
SN - 0920-9069
IS - 2-3
ER -