Involvement of IMP dehydrogenase activity in induction of sporulation of Bacillus cereus

Takahisa Miyamoto, Makoto Imamura, Kiyoshi Matsuno, Sam In Kim, Ken-ichi Honjoh, Shoji Hatano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

IMP dehydrogenase activity of B. cereus increased parallel to cell growth in YE-EMM, where B. cereus did not sporulate. When B. cereus was cultured in a modified G medium, a sporulation medium, the activity reached the highest level at 6 hr and decreased thereafter. After induction of sporulation by nutritional shift down in 1/100 G medium, the enzyme activity decreased to about 5% compared with exponentially growing cells at 1 hr of resuspension. The sporulation rate of B. cereus was over 90% in the modified G medium and 1/100 G medium. Sporulation was strongly inhibited by mycophenolic acid at 1 mM, when the drug was added at 0 and 1 hr of resuspension in 1/100 G medium. Intracellular GTP concentration of B. cereus decreased to the lowest level about 1 hr of resuspension. Although GTP increased to about 50% of the exponentially growing cells at 2 hr of resuspension in control cells, the concentration did not increase in the presence of 1 mM mycophenolic acid.

Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalMicrobiological Research
Volume152
Issue number3
DOIs
Publication statusPublished - Jan 1 1997

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IMP Dehydrogenase
Bacillus cereus
Mycophenolic Acid
Guanosine Triphosphate
Enzymes
Growth
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Involvement of IMP dehydrogenase activity in induction of sporulation of Bacillus cereus. / Miyamoto, Takahisa; Imamura, Makoto; Matsuno, Kiyoshi; Kim, Sam In; Honjoh, Ken-ichi; Hatano, Shoji.

In: Microbiological Research, Vol. 152, No. 3, 01.01.1997, p. 277-280.

Research output: Contribution to journalArticle

Miyamoto, Takahisa ; Imamura, Makoto ; Matsuno, Kiyoshi ; Kim, Sam In ; Honjoh, Ken-ichi ; Hatano, Shoji. / Involvement of IMP dehydrogenase activity in induction of sporulation of Bacillus cereus. In: Microbiological Research. 1997 ; Vol. 152, No. 3. pp. 277-280.
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