Involvement of interleukin-8, vascular endothelial growth factor, and basic fibroblast growth factor in tumor necrosis factor alpha-dependent angiogenesis

Shigeo Yoshida, Mayumi Ono, Tadahisa Shono, Hiroto Izumi, Tatsuro Ishibashi, Hideo Suzuki, Michihiko Kuwano

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Abstract

Tumor necrosis factor alpha (TNF-α) is a macrophage/monocyte-derived polypeptide which modulates the expression of various genes in vascular endothelial cells and induces angiogenesis. However, the underlying mechanism by which TNF-α mediates angiogenesis is not completely understood. In this study, we assessed whether TNF-α-induced angiogenesis is mediated through TNF-α itself or indirectly through other TNF-α-induced angiogenesis- promoting factors. Cellular mRNA levels of interleukin-8 (IL-8), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and their receptors were increased after the treatment of human microvascular endothelial cells with TNF-α (100 U/ml). TNF-α-dependent tubular morphogenesis in vascular endothelial cells was inhibited by the administration of anti-IL-8, anti-VEGF, and anti-bFGF antibodies, and coadministration of all three antibodies almost completely abrogated tubular formation. Moreover, treatment with Sp1, NF-κB, and c-Jun antisense oligonucleotides inhibited TNF-α-dependent tubular morphogenesis by microvascular endothelial cells. Administration of a NF-κB antisense oligonucleotide almost completely inhibited TNF-α-dependent IL-8 production and partially abrogated TNF-α-dependent VEGF production, and an Sp1 antisense sequence partially inhibited TNF-α-dependent production of VEGF. A c-Jun antisense oligonucleotide significantly inhibited TNF-α-dependent bFGF production but did not affect the production of IL-8 and VEGF. Administration of an anti-IL-8 or anti-VEGF antibody also blocked TNF-α-induced neovascularization in the rabbit cornea in vivo. Thus, angiogenesis by TNF- α appears to be modulated through various angiogenic factors, both in vitro and in vivo, and this pathway is controlled through paracrine and/or autocrine mechanisms.

Original languageEnglish
Pages (from-to)4015-4023
Number of pages9
JournalMolecular and cellular biology
Volume17
Issue number7
DOIs
Publication statusPublished - Jul 1997

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Fibroblast Growth Factor 2
Interleukin-8
Vascular Endothelial Growth Factor A
Tumor Necrosis Factor-alpha
Antisense Oligonucleotides
Endothelial Cells
Angiogenesis Inducing Agents
Morphogenesis
Antibodies
Fibroblast Growth Factor Receptors
Cornea

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Involvement of interleukin-8, vascular endothelial growth factor, and basic fibroblast growth factor in tumor necrosis factor alpha-dependent angiogenesis. / Yoshida, Shigeo; Ono, Mayumi; Shono, Tadahisa; Izumi, Hiroto; Ishibashi, Tatsuro; Suzuki, Hideo; Kuwano, Michihiko.

In: Molecular and cellular biology, Vol. 17, No. 7, 07.1997, p. 4015-4023.

Research output: Contribution to journalArticle

Yoshida, Shigeo ; Ono, Mayumi ; Shono, Tadahisa ; Izumi, Hiroto ; Ishibashi, Tatsuro ; Suzuki, Hideo ; Kuwano, Michihiko. / Involvement of interleukin-8, vascular endothelial growth factor, and basic fibroblast growth factor in tumor necrosis factor alpha-dependent angiogenesis. In: Molecular and cellular biology. 1997 ; Vol. 17, No. 7. pp. 4015-4023.
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