Involvement of intracellular cAMP in epirubicin-induced vascular endothelial cell injury

Takaaki Yamada, Mitsuyo Ueda, Nobuaki Egashira, Nina Zukeyama, Jun Kuwahara, Satohiro Masuda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We investigated the involvement of intracellular cAMP in endothelial cell injury induced by epirubicin. Epirubicin-induced decrease in cell viability and increase in caspase-3/7 activity were reversed by a cAMP analog dibutyryl cAMP (DBcAMP) or an activator of adenylate cyclase forskolin concomitant with a phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine. Moreover, epirubicin-induced elevation of lipid peroxide levels was attenuated by DBcAMP. Interestingly, the exposure of epirubicin decreased intracellular cAMP levels before the onset of epirubicin-induced production of lipid peroxidation. These results suggest that intracellular cAMP plays an important role in epirubicin-induced endothelial cell injury.

Original languageEnglish
Pages (from-to)33-37
Number of pages5
JournalJournal of Pharmacological Sciences
Volume130
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

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All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

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