Involvement of mTOR in globular adiponectin-induced generation of reactive oxygen species

Akie Fujimoto, Sumio Akifusa, Noriaki Kamio, Takao Hirofuji, Kazuaki Nonaka, Yoshihisa Yamashita

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated the role of the mammalian target of rapamycin (mTOR) in gAd-induced ROS and NO generation. gAd stimulation induced phosphorylation of mTOR, which peaked at 20 min and dissolved rapidly. Inhibition of phosphatidylinositol 3-kinase activity with wortmannin suppressed gAd-induced phosphorylation of Akt and mTOR. Administration of rapamycin partially reduced gAd-induced generation of intracellular and mitochondrial ROS, but not release of NO. To further confirm the role of mTOR in gAd stimulation, the effect of the activators of AMP-activated protein kinase (AMPK) on gAd-induced mTOR phosphorylation was examined. Pre-treatment with three kinds of AMPK activators, AICAR, 2-deoxy-D-glucose and A-769662, suppressed gAd-induced mTOR phosphorylation. Furthermore, these AMPK activators significantly reduced gAd-evoked intracellular and mitochondrial ROS generation and NO release.

Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalFree Radical Research
Volume44
Issue number2
DOIs
Publication statusPublished - Jan 25 2010

All Science Journal Classification (ASJC) codes

  • Biochemistry

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