Involvement of p27KIP1 degradation by SKp2 in the regulation of proliferation in response to wounding of corneal epithelium

Kazuhiko Yoshida, Keiko Nakayama, Hiroyasu Nagahama, Takayuki Harada, Chikako Harada, Junko Imaki, Akira Matsuda, Kazuyuki Yamamoto, Miyuki Ito, Shigeaki Ohno, Keiichi Nakayama

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

PURPOSE. To examine the expression of the p27KIP1 in the normal and epithelial-scraped cornea and whether degradation of p27KIP1 by Skp2 is involved in the regulation of cell proliferation in response to wounding of the corneal epithelium. METHODS. C57B16, p27KIP1-/-, Skp2-/-, and Skp2-/-/p27KIP1-/- double-knockout mice were examined. Normal and epithelial-scraped corneas were analyzed by immunocytochemistry using anti-p27KIP1 antibody. Cells in the S phase of DNA synthesis were analyzed by immunocytochemistry using anti-bromodeoxyuridine (BrdU) antibody. RESULTS. The p27KIP1 was expressed in basal cells of the central and peripheral region of the cornea and limbus. This expression was not detected 24 hours after the epithelial scraping, when there were many cells in the S phase of DNA synthesis in the corneal epithelium. There were no obvious differences in the thickness and anti-BrdU staining in the corneal epithelium of p27KIP1-/- mice from that of control animals. Twenty-four hours after epithelial scraping in the Skp2-/- mice, the corneal epithelium was thinner than in wild-type mice and had many p27KIP1-positive cells and few BrdU-positive cells. In contrast, 24 hours after epithelial scraping in the Skp2-/- /p27KIP1-/- double-knockout mice, the corneal epithelium was as thick as in wild-type mice and had many BrdU-positive cells. CONCLUSIONS. These results suggest that degradation of p27KIP1 by Skp2 is involved in the regulation of cell proliferation in response to wounding of the corneal epithelium.

Original languageEnglish
Pages (from-to)364-370
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume43
Issue number2
Publication statusPublished - Feb 11 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Involvement of p27<sup>KIP1</sup> degradation by SKp2 in the regulation of proliferation in response to wounding of corneal epithelium'. Together they form a unique fingerprint.

Cite this