Involvement of periostin in regression of hyaloidvascular system during ocular development

Mitsuru Arima, Shigeo Yoshida, Takahito Nakama, Keijiro Ishikawa, Shintaro Nakao, Takeru Yoshimura, Ryo Asato, Yukio Sassa, Takeshi Kita, Hiroshi Enaida, Yuji Oshima, Akira Matsuda, Akira Kudo, Tatsuro Ishibashi

Research output: Contribution to journalArticle

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Abstract

Purpose. A timely regression of the hyaloid vascular system (HVS) is required for the normal ocular development. Although macrophages have a critical role in this process, the exact mechanism remains undetermined. Periostin is a matricellular protein involved in tissue and vascular remodeling. The purpose of our study was to determine whether periostin is involved in the HVS regression. Methods. We used wild type (WT) and periostin knockout (KO) mice. Indocyanine green angiography and immunohistochemistry with isolectin B4 were used to evaluate the HVS regression. TUNEL-labeling was used to quantify the number of apoptotic hyaloid vascular endothelial cells. F4/80 and Iba-1 staining was performed to determine the number and location of macrophages in the vitreous. The location of periostin also was investigated by immunohistochemistry. To determine the functional role of periostin, the degree of adhesion of human monocytes to fibronectin was measured by an adhesion assay. Results. The HVS regression and peak in the number of TUNEL-positive apoptotic endothelial cells were delayed in periostin KO mice. The number of F4/80 positive cells in the vitreous was higher in periostin KO mice. Only a small number of Iba-1-positive cells near the hyaloid vessels was co-stained with periostin, and peripheral blood monocytes were not stained with periostin. Adhesion assay showed that periostin increased the degree of attachment of monocytes to fibronectin. Conclusions. These results suggest that periostin, which is secreted by the intraocular macrophages, enhances the HVS regression by intensifying the adhesion of macrophages to hyaloid vessels.

Original languageEnglish
Pages (from-to)6495-6503
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number10
DOIs
Publication statusPublished - Sep 1 2012

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Blood Vessels
Macrophages
Knockout Mice
Monocytes
In Situ Nick-End Labeling
Fibronectins
Endothelial Cells
Immunohistochemistry
Indocyanine Green
Lectins
Angiography
Staining and Labeling
Proteins

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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Involvement of periostin in regression of hyaloidvascular system during ocular development. / Arima, Mitsuru; Yoshida, Shigeo; Nakama, Takahito; Ishikawa, Keijiro; Nakao, Shintaro; Yoshimura, Takeru; Asato, Ryo; Sassa, Yukio; Kita, Takeshi; Enaida, Hiroshi; Oshima, Yuji; Matsuda, Akira; Kudo, Akira; Ishibashi, Tatsuro.

In: Investigative Ophthalmology and Visual Science, Vol. 53, No. 10, 01.09.2012, p. 6495-6503.

Research output: Contribution to journalArticle

Arima, M, Yoshida, S, Nakama, T, Ishikawa, K, Nakao, S, Yoshimura, T, Asato, R, Sassa, Y, Kita, T, Enaida, H, Oshima, Y, Matsuda, A, Kudo, A & Ishibashi, T 2012, 'Involvement of periostin in regression of hyaloidvascular system during ocular development', Investigative Ophthalmology and Visual Science, vol. 53, no. 10, pp. 6495-6503. https://doi.org/10.1167/iovs.12-9684
Arima, Mitsuru ; Yoshida, Shigeo ; Nakama, Takahito ; Ishikawa, Keijiro ; Nakao, Shintaro ; Yoshimura, Takeru ; Asato, Ryo ; Sassa, Yukio ; Kita, Takeshi ; Enaida, Hiroshi ; Oshima, Yuji ; Matsuda, Akira ; Kudo, Akira ; Ishibashi, Tatsuro. / Involvement of periostin in regression of hyaloidvascular system during ocular development. In: Investigative Ophthalmology and Visual Science. 2012 ; Vol. 53, No. 10. pp. 6495-6503.
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abstract = "Purpose. A timely regression of the hyaloid vascular system (HVS) is required for the normal ocular development. Although macrophages have a critical role in this process, the exact mechanism remains undetermined. Periostin is a matricellular protein involved in tissue and vascular remodeling. The purpose of our study was to determine whether periostin is involved in the HVS regression. Methods. We used wild type (WT) and periostin knockout (KO) mice. Indocyanine green angiography and immunohistochemistry with isolectin B4 were used to evaluate the HVS regression. TUNEL-labeling was used to quantify the number of apoptotic hyaloid vascular endothelial cells. F4/80 and Iba-1 staining was performed to determine the number and location of macrophages in the vitreous. The location of periostin also was investigated by immunohistochemistry. To determine the functional role of periostin, the degree of adhesion of human monocytes to fibronectin was measured by an adhesion assay. Results. The HVS regression and peak in the number of TUNEL-positive apoptotic endothelial cells were delayed in periostin KO mice. The number of F4/80 positive cells in the vitreous was higher in periostin KO mice. Only a small number of Iba-1-positive cells near the hyaloid vessels was co-stained with periostin, and peripheral blood monocytes were not stained with periostin. Adhesion assay showed that periostin increased the degree of attachment of monocytes to fibronectin. Conclusions. These results suggest that periostin, which is secreted by the intraocular macrophages, enhances the HVS regression by intensifying the adhesion of macrophages to hyaloid vessels.",
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AU - Arima, Mitsuru

AU - Yoshida, Shigeo

AU - Nakama, Takahito

AU - Ishikawa, Keijiro

AU - Nakao, Shintaro

AU - Yoshimura, Takeru

AU - Asato, Ryo

AU - Sassa, Yukio

AU - Kita, Takeshi

AU - Enaida, Hiroshi

AU - Oshima, Yuji

AU - Matsuda, Akira

AU - Kudo, Akira

AU - Ishibashi, Tatsuro

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N2 - Purpose. A timely regression of the hyaloid vascular system (HVS) is required for the normal ocular development. Although macrophages have a critical role in this process, the exact mechanism remains undetermined. Periostin is a matricellular protein involved in tissue and vascular remodeling. The purpose of our study was to determine whether periostin is involved in the HVS regression. Methods. We used wild type (WT) and periostin knockout (KO) mice. Indocyanine green angiography and immunohistochemistry with isolectin B4 were used to evaluate the HVS regression. TUNEL-labeling was used to quantify the number of apoptotic hyaloid vascular endothelial cells. F4/80 and Iba-1 staining was performed to determine the number and location of macrophages in the vitreous. The location of periostin also was investigated by immunohistochemistry. To determine the functional role of periostin, the degree of adhesion of human monocytes to fibronectin was measured by an adhesion assay. Results. The HVS regression and peak in the number of TUNEL-positive apoptotic endothelial cells were delayed in periostin KO mice. The number of F4/80 positive cells in the vitreous was higher in periostin KO mice. Only a small number of Iba-1-positive cells near the hyaloid vessels was co-stained with periostin, and peripheral blood monocytes were not stained with periostin. Adhesion assay showed that periostin increased the degree of attachment of monocytes to fibronectin. Conclusions. These results suggest that periostin, which is secreted by the intraocular macrophages, enhances the HVS regression by intensifying the adhesion of macrophages to hyaloid vessels.

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