Involvement of platelet-derived growth factor receptor β in fibrosis through extracellular matrix protein production after ischemic stroke

Noriko Makihara, Koichi Arimura, Tetsuro Ago, Masaki Tachibana, Ataru Nishimura, Kuniyuki Nakamura, Ryu Matsuo, Yoshinobu Wakisaka, Junya Kuroda, Hiroshi Sugimori, Masahiro Kamouchi, Takanari Kitazono

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Abstract

Fibrosis is concomitant with repair processes following injuries in the central nervous system (CNS). Pericytes are considered as an origin of fibrosis-forming cells in the CNS. Here, we examined whether platelet-derived growth factor receptor β (PDGFRβ), a well-known indispensable molecule for migration, proliferation, and survival of pericytes, was involved in the production of extracellular matrix proteins, fibronectin and collagen type I, which is crucial for fibrosis after ischemic stroke. Immunohistochemistry demonstrated induction of PDGFRβ expression in vascular cells of peri-infarct areas at 3-7. days in a mouse stroke model. The PDGFRβ-expressing cells extended from peri-infarct areas toward the ischemic core after day 7 while expressing fibronectin and collagen type I in the infarct areas. In contrast, desmin and α-smooth muscle actin, markers of pericytes, were only expressed in vascular cells. In PDGFRβ heterozygous knockout mice, the expression of fibronectin and collagen type I was attenuated at both mRNA and protein levels with an enlargement of the infarct volume after ischemic stroke compared with that in wild-type littermates. In cultured brain pericytes, the expression of PDGF-B, PDGFRβ, fibronectin, and collagen type I, but not desmin, was significantly increased by serum depletion (SD). The SD-induced upregulation of fibronectin and collagen type I was suppressed by SU11652, an inhibitor of PDGFRβ, while PDGF-B further increased the SD-induced upregulation. In conclusion, the expression level of PDGFRβ may be a crucial determinant of fibrosis after ischemic stroke. Moreover, PDGFRβ signaling participates in the production of fibronectin and collagen type I after ischemic stroke.

Original languageEnglish
Pages (from-to)127-134
Number of pages8
JournalExperimental Neurology
Volume264
DOIs
Publication statusPublished - Feb 1 2015

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Platelet-Derived Growth Factor Receptors
Extracellular Matrix Proteins
Fibrosis
Collagen Type I
Stroke
Fibronectins
Pericytes
Desmin
Blood Vessels
Up-Regulation
Central Nervous System
Serum
Knockout Mice
Smooth Muscle
Actins
Immunohistochemistry
Messenger RNA
Wounds and Injuries
Brain

All Science Journal Classification (ASJC) codes

  • Neurology
  • Developmental Neuroscience

Cite this

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title = "Involvement of platelet-derived growth factor receptor β in fibrosis through extracellular matrix protein production after ischemic stroke",
abstract = "Fibrosis is concomitant with repair processes following injuries in the central nervous system (CNS). Pericytes are considered as an origin of fibrosis-forming cells in the CNS. Here, we examined whether platelet-derived growth factor receptor β (PDGFRβ), a well-known indispensable molecule for migration, proliferation, and survival of pericytes, was involved in the production of extracellular matrix proteins, fibronectin and collagen type I, which is crucial for fibrosis after ischemic stroke. Immunohistochemistry demonstrated induction of PDGFRβ expression in vascular cells of peri-infarct areas at 3-7. days in a mouse stroke model. The PDGFRβ-expressing cells extended from peri-infarct areas toward the ischemic core after day 7 while expressing fibronectin and collagen type I in the infarct areas. In contrast, desmin and α-smooth muscle actin, markers of pericytes, were only expressed in vascular cells. In PDGFRβ heterozygous knockout mice, the expression of fibronectin and collagen type I was attenuated at both mRNA and protein levels with an enlargement of the infarct volume after ischemic stroke compared with that in wild-type littermates. In cultured brain pericytes, the expression of PDGF-B, PDGFRβ, fibronectin, and collagen type I, but not desmin, was significantly increased by serum depletion (SD). The SD-induced upregulation of fibronectin and collagen type I was suppressed by SU11652, an inhibitor of PDGFRβ, while PDGF-B further increased the SD-induced upregulation. In conclusion, the expression level of PDGFRβ may be a crucial determinant of fibrosis after ischemic stroke. Moreover, PDGFRβ signaling participates in the production of fibronectin and collagen type I after ischemic stroke.",
author = "Noriko Makihara and Koichi Arimura and Tetsuro Ago and Masaki Tachibana and Ataru Nishimura and Kuniyuki Nakamura and Ryu Matsuo and Yoshinobu Wakisaka and Junya Kuroda and Hiroshi Sugimori and Masahiro Kamouchi and Takanari Kitazono",
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T1 - Involvement of platelet-derived growth factor receptor β in fibrosis through extracellular matrix protein production after ischemic stroke

AU - Makihara, Noriko

AU - Arimura, Koichi

AU - Ago, Tetsuro

AU - Tachibana, Masaki

AU - Nishimura, Ataru

AU - Nakamura, Kuniyuki

AU - Matsuo, Ryu

AU - Wakisaka, Yoshinobu

AU - Kuroda, Junya

AU - Sugimori, Hiroshi

AU - Kamouchi, Masahiro

AU - Kitazono, Takanari

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Fibrosis is concomitant with repair processes following injuries in the central nervous system (CNS). Pericytes are considered as an origin of fibrosis-forming cells in the CNS. Here, we examined whether platelet-derived growth factor receptor β (PDGFRβ), a well-known indispensable molecule for migration, proliferation, and survival of pericytes, was involved in the production of extracellular matrix proteins, fibronectin and collagen type I, which is crucial for fibrosis after ischemic stroke. Immunohistochemistry demonstrated induction of PDGFRβ expression in vascular cells of peri-infarct areas at 3-7. days in a mouse stroke model. The PDGFRβ-expressing cells extended from peri-infarct areas toward the ischemic core after day 7 while expressing fibronectin and collagen type I in the infarct areas. In contrast, desmin and α-smooth muscle actin, markers of pericytes, were only expressed in vascular cells. In PDGFRβ heterozygous knockout mice, the expression of fibronectin and collagen type I was attenuated at both mRNA and protein levels with an enlargement of the infarct volume after ischemic stroke compared with that in wild-type littermates. In cultured brain pericytes, the expression of PDGF-B, PDGFRβ, fibronectin, and collagen type I, but not desmin, was significantly increased by serum depletion (SD). The SD-induced upregulation of fibronectin and collagen type I was suppressed by SU11652, an inhibitor of PDGFRβ, while PDGF-B further increased the SD-induced upregulation. In conclusion, the expression level of PDGFRβ may be a crucial determinant of fibrosis after ischemic stroke. Moreover, PDGFRβ signaling participates in the production of fibronectin and collagen type I after ischemic stroke.

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