Involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland

Kouhei Hayashi, Tatsuya Ikari, Goro Sugiyama, Tsuyoshi Sugiura, Yukiko Ohyama, Wataru Kumamaru, Kanemitsu Shirasuna, Yoshihide Mori

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The mouse submandibular gland (SMG) is important organ for embryonic development, and branching morphogenesis is regulated by many molecules containing transcription factors. Real-time reverse transcriptase polymerase chain reaction revealed that the expression of Brachyury increased in the SMG and peaked between E12.5–E13.5, concomitant with the early stage of branching morphogenesis. The expression of Brachyury in SMG rudiments between E12.5–E13.5 was confirmed by western blotting. In addition, fibronectin and Btbd7 (regulated by fibronectin), which are both essential for cleft formation, were expressed strongly during the same period. The Sox2 and Wnt3a, which regulate cell growth, were also expressed strongly during E12.5–E13.5. On the other hand, cleft formation and branching morphogenesis was suppressed by knockdown of Brachyury gene, suggesting that Brachyury plays a central role in regulating cell growth and cleft formation in early-stage embryonic mouse salivary gland development.

Original languageEnglish
Pages (from-to)814-819
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume477
Issue number4
DOIs
Publication statusPublished - Sep 2 2016

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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