Involvement of thyrotropin in photoperiodic signal transduction in mice

Hiroko Ono, Yuta Hoshino, Shinobu Yasuo, Miwa Watanabe, Yusuke Nakane, Atsushi Murai, Shizufumi Ebihara, Horst Werner Korf, Takashi Yoshimura

Research output: Contribution to journalArticle

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Abstract

Local thyroid hormone catabolism within the mediobasal hypothalamus (MBH) by thyroid hormone-activating (DIO2) and -inactivating (DIO3) enzymes regulates seasonal reproduction in birds and mammals. Recent functional genomics analysis in birds has shown that long days induce thyroid-stimulating hormone production in the pars tuberalis (PT) of the pituitary gland, which triggers DIO2 expression in the ependymal cells (EC) of the MBH. In mammals, nocturnal melatonin secretion provides an endocrine signal of the photoperiod to the PT that contains melatonin receptors in high density, but the interface between the melatonin signal perceived in the PT and the thyroid hormone levels in the MBH remains unclear. Here we provide evidence in mice that TSH participates in this photoperiodic signal transduction. Although most mouse strains are considered to be nonseasonal, a robust photoperiodic response comprising induced expression of TSHB (TSH β subunit), CGA (TSH α subunit), and DIO2, and reduced expression of DIO3, was observed in melatonin-proficient CBA/N mice. These responses could not be elicited in melatonin-deficient C57BL/6J, but treatment of C57BL/6J mice with exogenous melatonin elicited similar effects on the expression of the above-mentioned genes as observed in CBA/N after transfer to short-day conditions. The EC was found to express TSH receptor (TSHR), and ICV injection of TSH induced DIO2 expression. Finally, we show that melatonin administration did not affect the expression of TSHB, DIO2, and DIO3 in TSHR-null mice. Taken together, our findings suggest that melatonin-dependent regulation of thyroid hormone levels in the MBH appears to involve TSH in mammals.

Original languageEnglish
Pages (from-to)18238-18242
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number47
DOIs
Publication statusPublished - Nov 25 2008

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Thyrotropin
Melatonin
Signal Transduction
Thyroid Hormones
Hypothalamus
Mammals
Thyrotropin Receptors
Birds
Melatonin Receptors
Inbred CBA Mouse
Photoperiod
Pituitary Gland
Genomics
Inbred C57BL Mouse
Reproduction
Injections
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Involvement of thyrotropin in photoperiodic signal transduction in mice. / Ono, Hiroko; Hoshino, Yuta; Yasuo, Shinobu; Watanabe, Miwa; Nakane, Yusuke; Murai, Atsushi; Ebihara, Shizufumi; Korf, Horst Werner; Yoshimura, Takashi.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 47, 25.11.2008, p. 18238-18242.

Research output: Contribution to journalArticle

Ono, H, Hoshino, Y, Yasuo, S, Watanabe, M, Nakane, Y, Murai, A, Ebihara, S, Korf, HW & Yoshimura, T 2008, 'Involvement of thyrotropin in photoperiodic signal transduction in mice', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 47, pp. 18238-18242. https://doi.org/10.1073/pnas.0808952105
Ono, Hiroko ; Hoshino, Yuta ; Yasuo, Shinobu ; Watanabe, Miwa ; Nakane, Yusuke ; Murai, Atsushi ; Ebihara, Shizufumi ; Korf, Horst Werner ; Yoshimura, Takashi. / Involvement of thyrotropin in photoperiodic signal transduction in mice. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 47. pp. 18238-18242.
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