Involvement of Wiskott-Aldrich Syndrome Protein (WASP) in B cell antigen receptor and Btk signaling pathway

Yoshihiro Baba, S. Nonoyama, K. Imai, H. D. Ochs, J. Yata, T. Kishimoto

Research output: Contribution to journalArticle

Abstract

Bruton's tyrosine kinase (Btk) is a cytoplasmic tyrosine kinase which is involved in the pathogenesis of human and murine B cell deficiencies. In this study, we attempted to identify the downstream molecule in the Btk signaling pathway. We showed that WASP physically associated with Btk in B-lineage cells and was constitutively phosphorylated on its tyrosine in a preB cell line in which the tyrosine-phosphorylation of Btk was also prominent WASP was shown to be phosphorylated by Btk in cotransfection system. The phosphotyrosine motif of WASP was distinctly similar to that of the autophosphorylation site of Btk. Furthermore, WASP was shown to be tyrosine-phosphorylated upon B cell antigen receptor crosslinking. These results indicate a physical and functional link of Btk and WASP, and also suggest a possible involvement of WASP in the antigen receptor signaling pathway in B-lineage cells together with Btk.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
Publication statusPublished - Mar 20 1998

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Wiskott-Aldrich Syndrome Protein
B-Cell Antigen Receptors
Tyrosine
Cells
Phosphotyrosine
Antigen Receptors
Phosphorylation
Agammaglobulinaemia tyrosine kinase
IgA receptor
Crosslinking
Protein-Tyrosine Kinases
B-Lymphocytes
Cell Line

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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Involvement of Wiskott-Aldrich Syndrome Protein (WASP) in B cell antigen receptor and Btk signaling pathway. / Baba, Yoshihiro; Nonoyama, S.; Imai, K.; Ochs, H. D.; Yata, J.; Kishimoto, T.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Baba, Yoshihiro ; Nonoyama, S. ; Imai, K. ; Ochs, H. D. ; Yata, J. ; Kishimoto, T. / Involvement of Wiskott-Aldrich Syndrome Protein (WASP) in B cell antigen receptor and Btk signaling pathway. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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AU - Yata, J.

AU - Kishimoto, T.

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