Ion channels and second messengers involved in transduction and modulation of sweet taste in mouse taste cells

Kumiko Sugimoto, Noriatsu Shigemura, Keiko Nakano, Rie Ohta, Kiyohito Nakashima, Kirio Kawai, Yuzo Ninomiya

Research output: Contribution to journalArticle

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Abstract

Leptin, a hormone released from the adipose tissue, inhibits food intake and increases energy expenditure. We have found a novel function of leptin as a modulator of sweet taste sensitivity in mice. In lean normal mice, the gustatory nerve responses to sweet stimuli were selectively suppressed depending on plasma leptin level after an intraperitoneal injection of recombinant leptin. Patch-clamp studies using isolated taste cells of lean mice showed that extracellular leptin enhanced K+ currents of sweet-responsive taste cells, which led to membrane hyperpolarization and a reduction of sweetener-induced depolarization. Reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization analyses demonstrated specific expression of mRNA of the long-form functional leptin receptor (Ob-Rb) in taste tissue and cells of lean mice. The genetically diabetic db/db mice, which have defects in Ob-Rb, demonstrated neither a suppression of gustatory neural responses to sweeteners nor an increment of whole-cell K+ conductance of taste cells even with high doses of leptin. These results suggest that Ob-Rb is specifically expressed in sweet-responsive taste cells of lean mice and that leptin suppresses sweetener-induced depolarization via activation of K+ channels, leading to a decrease in impulses of sweet-best fibers. The enhanced sweet responses of db/db mice may result from the lack of inhibitory modulation by leptin.

Original languageEnglish
Pages (from-to)1141-1151
Number of pages11
JournalPure and Applied Chemistry
Volume74
Issue number7
DOIs
Publication statusPublished - Jul 1 2002

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Second Messenger Systems
Leptin
Ion Channels
Cells
Depolarization
Modulation
Ions
Sweetening Agents
Tissue
Polymerase chain reaction
Hormones
Clamping devices
Transcription
Modulators
Chemical activation
Membranes
Plasmas
Defects
Fibers
Leptin Receptors

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Ion channels and second messengers involved in transduction and modulation of sweet taste in mouse taste cells. / Sugimoto, Kumiko; Shigemura, Noriatsu; Nakano, Keiko; Ohta, Rie; Nakashima, Kiyohito; Kawai, Kirio; Ninomiya, Yuzo.

In: Pure and Applied Chemistry, Vol. 74, No. 7, 01.07.2002, p. 1141-1151.

Research output: Contribution to journalArticle

Sugimoto, Kumiko ; Shigemura, Noriatsu ; Nakano, Keiko ; Ohta, Rie ; Nakashima, Kiyohito ; Kawai, Kirio ; Ninomiya, Yuzo. / Ion channels and second messengers involved in transduction and modulation of sweet taste in mouse taste cells. In: Pure and Applied Chemistry. 2002 ; Vol. 74, No. 7. pp. 1141-1151.
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