Ion selectivity of Ba2+ inward current oscillations in ras-transformed fibroblasts that elicit cytoplasmic Ca2+ oscillations by bradykinin

H. Higashida, M. Shahidullah, N. Hoshi, M. Noda, M. Hashii, Z. G. Zhong, Y. Nozawa

Research output: Contribution to journalArticle

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Abstract

Ion selectivity of divalent cations on Ba2+ inward current oscillations was examined by voltage-clamp recording in ν-Ki-ras-transformed NIH/3T3 (DT) fibroblasts where repetitive transient increases in cytoplasmic Ca2+ concentration were evoked by bradykinin. Application of bradykinin onto DT cells in 50 mM Ba2+ solution initiated Ba2+ inward current oscillations. The inward currents were inhibited in equimolar Sr2+ or Ca2+ solutions. Ba2+ current oscillations were dependent upon extracellular Ba2+ concentration. The results suggest that inward current oscillations are highly selective to Ba2+.

Original languageEnglish
Pages (from-to)162-166
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume185
Issue number1
DOIs
Publication statusPublished - May 29 1992
Externally publishedYes

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Bradykinin
Fibroblasts
Ions
Divalent Cations
Clamping devices
Electric potential

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ion selectivity of Ba2+ inward current oscillations in ras-transformed fibroblasts that elicit cytoplasmic Ca2+ oscillations by bradykinin. / Higashida, H.; Shahidullah, M.; Hoshi, N.; Noda, M.; Hashii, M.; Zhong, Z. G.; Nozawa, Y.

In: Biochemical and Biophysical Research Communications, Vol. 185, No. 1, 29.05.1992, p. 162-166.

Research output: Contribution to journalArticle

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