Ionic currents underlying rhythmic bursting of ventral mossy cells in the developing mouse dentate gyrus

Shozo Jinno, Satoru Ishizuka, Toshio Kosaka

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

The electrophysiological properties of mossy cells were examined in developing mouse hippocampal slices using whole-cell patch-clamp techniques, with particular reference to the dorsoventral difference. Dorsal mossy cells exhibited a higher spontaneous excitatory postsynaptic potential (EPSP) frequency and larger maximal EPSP amplitude than ventral mossy cells. On the other hand, the blockade of synaptic inputs with glutamatergic and GABAergic antagonists disclosed a remarkable dorsoventral difference in the intrinsic activity: none (0/27) of the dorsal mossy cells showed intrinsic bursting, whereas the majority (35/47) of the ventral mossy cells exhibited intrinsic rhythmic bursting. To characterize the ionic currents underlying the rhythmic bursting of mossy cells, we used somatic voltage-clamp recordings in the subthreshold voltage range. Ventral bursting cells possessed both hyperpolarization-activated current (/h) and persistent sodium current (/NaP), whereas dorsal and ventral nonbursting cells possessed /h but no /NaP. Blockade of /h with cesium did not affect the intrinsic bursting of ventral mossy cells. In contrast, the blockade of /NaP with tetrodotoxin or phenytoin established a stable subthreshold membrane potential in ventral bursting cells. The current-voltage curve of ventral bursting cells showed a region of tetrodotoxin-sensitive negative slope conductance between -55 mV and a spike threshold (≈ -45 mV). On the other hand, no subthreshold calcium conductances played a significant role in the intrinsic bursting of ventral mossy cells. These observations demonstrate the heterogeneous electrophysiological properties of hilar mossy cells, and suggest that the subthreshold /NaP plays a major role in the intrinsic rhythmic bursting of ventral mossy cells.

Original languageEnglish
Pages (from-to)1338-1354
Number of pages17
JournalEuropean Journal of Neuroscience
Volume17
Issue number7
DOIs
Publication statusPublished - Apr 1 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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