Ionic liquids induced structural changes of bovine serum albumin in aqueous media: A detailed physicochemical and spectroscopic study

Tejwant Singh, Pankaj Bharmoria, Masa Aki Morikawa, Nobuo Kimizuka, Arvind Kumar

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Structural changes of a globular protein, bovine serum albumin (BSA), as a consequence of interaction with the surface active ionic liquids (ILs)-3-methyl-1-octylimidazolium chloride, [C8mim][Cl], and 1-butyl-3-methylimidazolium octylsulfate, [C4mim][C 8OSO3]-have been investigated using various physicochemical and spectroscopic techniques such as tensiometry, conductometry, steady-state fluorescence, far-UV circular dichroism spectroscopy (CD), and dynamic light scattering (DLS). The interactional behavior of ILs (monomers and self-assembled structures) toward BSA in different IL concentration regimes at the air/solution interface as well as in the bulk is investigated and discussed depending upon the nature of ions of ILs. CD combined with the steady state fluorescence spectroscopy provided valuable insights into the unfolding of BSA as a consequence of IL binding. The complementary results obtained from the multitechnique approach proved very useful in drawing out the mechanism of interaction between ILs and BSA in different IL concentration regimes.

Original languageEnglish
Pages (from-to)11924-11935
Number of pages12
JournalJournal of Physical Chemistry B
Volume116
Issue number39
DOIs
Publication statusPublished - Oct 4 2012

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Ionic Liquids
Bovine Serum Albumin
albumins
Ionic liquids
serums
liquids
Circular dichroism spectroscopy
dichroism
spectroscopy
fluorescence
Fluorescence spectroscopy
Dynamic light scattering
Ultraviolet spectroscopy
light scattering
monomers
Monomers
Fluorescence
chlorides
interactions
Ions

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Ionic liquids induced structural changes of bovine serum albumin in aqueous media : A detailed physicochemical and spectroscopic study. / Singh, Tejwant; Bharmoria, Pankaj; Morikawa, Masa Aki; Kimizuka, Nobuo; Kumar, Arvind.

In: Journal of Physical Chemistry B, Vol. 116, No. 39, 04.10.2012, p. 11924-11935.

Research output: Contribution to journalArticle

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