Abstract
To elucidate the role of the platelet-derived growth factor (PDGF)-B chain in the brain, we examined its expression in rat brains with focal ischemia. Focal ischemia was induced by permanent tandem occlusion of the middle cerebral and common carotid arteries in spontaneously hypertensive rats (SHRs). Northern analysis demonstrated that ischemia transiently increased mRNA expression of the PDGF-B chain, but not the PDGF-A chain, in the injured neocortex. The larger transcript (3.5 kb) of the B chain gradually increased to threefold by 16 h, whereas the smaller transcript (2.6 kb) of the B chain markedly increased sixfold by 4 h. Immunohistochemistry revealed enhanced immunoreactivity in the neurons in the infarct and in the periinfarct area from 16 h to days 4-7, with a peak at 24 h. Furthermore, the brain macrophages that accumulated in the infarct showed intense immunostaining in their perinuclear region from days 2 to 14, with a peak at days 5-6. The present study demonstrates that ischemia induces the expression of the PDGF-B chain, first in neurons and later in brain macrophages, and suggests an important role of the PDGF-B chain in the healing process of the injured brain.
| Original language | English |
|---|---|
| Pages (from-to) | 818-824 |
| Number of pages | 7 |
| Journal | Journal of Cerebral Blood Flow and Metabolism |
| Volume | 14 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jan 1 1994 |
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All Science Journal Classification (ASJC) codes
- Neurology
- Clinical Neurology
- Cardiology and Cardiovascular Medicine
Cite this
Ischemia induces the expression of the platelet-derived growth factor-B chain in neurons and brain macrophages in vivo. / Iihara, Koji; Sasahara, Masakiyo; Hashimoto, Nobuo; Uemura, Yoshihiko; Kikuchi, Haruhiko; Hazama, Fumitada.
In: Journal of Cerebral Blood Flow and Metabolism, Vol. 14, No. 5, 01.01.1994, p. 818-824.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ischemia induces the expression of the platelet-derived growth factor-B chain in neurons and brain macrophages in vivo
AU - Iihara, Koji
AU - Sasahara, Masakiyo
AU - Hashimoto, Nobuo
AU - Uemura, Yoshihiko
AU - Kikuchi, Haruhiko
AU - Hazama, Fumitada
PY - 1994/1/1
Y1 - 1994/1/1
N2 - To elucidate the role of the platelet-derived growth factor (PDGF)-B chain in the brain, we examined its expression in rat brains with focal ischemia. Focal ischemia was induced by permanent tandem occlusion of the middle cerebral and common carotid arteries in spontaneously hypertensive rats (SHRs). Northern analysis demonstrated that ischemia transiently increased mRNA expression of the PDGF-B chain, but not the PDGF-A chain, in the injured neocortex. The larger transcript (3.5 kb) of the B chain gradually increased to threefold by 16 h, whereas the smaller transcript (2.6 kb) of the B chain markedly increased sixfold by 4 h. Immunohistochemistry revealed enhanced immunoreactivity in the neurons in the infarct and in the periinfarct area from 16 h to days 4-7, with a peak at 24 h. Furthermore, the brain macrophages that accumulated in the infarct showed intense immunostaining in their perinuclear region from days 2 to 14, with a peak at days 5-6. The present study demonstrates that ischemia induces the expression of the PDGF-B chain, first in neurons and later in brain macrophages, and suggests an important role of the PDGF-B chain in the healing process of the injured brain.
AB - To elucidate the role of the platelet-derived growth factor (PDGF)-B chain in the brain, we examined its expression in rat brains with focal ischemia. Focal ischemia was induced by permanent tandem occlusion of the middle cerebral and common carotid arteries in spontaneously hypertensive rats (SHRs). Northern analysis demonstrated that ischemia transiently increased mRNA expression of the PDGF-B chain, but not the PDGF-A chain, in the injured neocortex. The larger transcript (3.5 kb) of the B chain gradually increased to threefold by 16 h, whereas the smaller transcript (2.6 kb) of the B chain markedly increased sixfold by 4 h. Immunohistochemistry revealed enhanced immunoreactivity in the neurons in the infarct and in the periinfarct area from 16 h to days 4-7, with a peak at 24 h. Furthermore, the brain macrophages that accumulated in the infarct showed intense immunostaining in their perinuclear region from days 2 to 14, with a peak at days 5-6. The present study demonstrates that ischemia induces the expression of the PDGF-B chain, first in neurons and later in brain macrophages, and suggests an important role of the PDGF-B chain in the healing process of the injured brain.
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U2 - 10.1038/jcbfm.1994.102
DO - 10.1038/jcbfm.1994.102
M3 - Article
VL - 14
SP - 818
EP - 824
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
SN - 0271-678X
IS - 5
ER -