Ischemia induces the expression of the platelet-derived growth factor-B chain in neurons and brain macrophages in vivo

Koji Iihara, Masakiyo Sasahara, Nobuo Hashimoto, Yoshihiko Uemura, Haruhiko Kikuchi, Fumitada Hazama

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Abstract

To elucidate the role of the platelet-derived growth factor (PDGF)-B chain in the brain, we examined its expression in rat brains with focal ischemia. Focal ischemia was induced by permanent tandem occlusion of the middle cerebral and common carotid arteries in spontaneously hypertensive rats (SHRs). Northern analysis demonstrated that ischemia transiently increased mRNA expression of the PDGF-B chain, but not the PDGF-A chain, in the injured neocortex. The larger transcript (3.5 kb) of the B chain gradually increased to threefold by 16 h, whereas the smaller transcript (2.6 kb) of the B chain markedly increased sixfold by 4 h. Immunohistochemistry revealed enhanced immunoreactivity in the neurons in the infarct and in the periinfarct area from 16 h to days 4-7, with a peak at 24 h. Furthermore, the brain macrophages that accumulated in the infarct showed intense immunostaining in their perinuclear region from days 2 to 14, with a peak at days 5-6. The present study demonstrates that ischemia induces the expression of the PDGF-B chain, first in neurons and later in brain macrophages, and suggests an important role of the PDGF-B chain in the healing process of the injured brain.

Original languageEnglish
Pages (from-to)818-824
Number of pages7
JournalJournal of Cerebral Blood Flow and Metabolism
Volume14
Issue number5
DOIs
Publication statusPublished - Jan 1 1994

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Proto-Oncogene Proteins c-sis
Ischemia
Macrophages
Neurons
Brain
Common Carotid Artery
Neocortex
Inbred SHR Rats
Immunohistochemistry
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Ischemia induces the expression of the platelet-derived growth factor-B chain in neurons and brain macrophages in vivo. / Iihara, Koji; Sasahara, Masakiyo; Hashimoto, Nobuo; Uemura, Yoshihiko; Kikuchi, Haruhiko; Hazama, Fumitada.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 14, No. 5, 01.01.1994, p. 818-824.

Research output: Contribution to journalArticle

Iihara, Koji ; Sasahara, Masakiyo ; Hashimoto, Nobuo ; Uemura, Yoshihiko ; Kikuchi, Haruhiko ; Hazama, Fumitada. / Ischemia induces the expression of the platelet-derived growth factor-B chain in neurons and brain macrophages in vivo. In: Journal of Cerebral Blood Flow and Metabolism. 1994 ; Vol. 14, No. 5. pp. 818-824.
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