Isolation and amino acid sequences of small tryptic peptides from the oxidized ala chain of ricin d

Makoto Kimura, Gunki Funatsu, Masaru Funatsu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Twelve tryptic peptides as well as free arginine were isolated from the performic acid-oxidized Ala chain of ricin D by gel filtration on Sephadex G-25 and Dowex 1×2 column chromatography followed by paper chromatography. Total number of the amino acid residues in these peptides accounted for 90 out of 263 residues in the Ala chain of ricin D. The amino acid sequences of nine peptides were determined by manual Ed man degradation.

Original languageEnglish
Pages (from-to)1733-1736
Number of pages4
JournalAgricultural and Biological Chemistry
Volume41
Issue number9
DOIs
Publication statusPublished - Sep 1977

Fingerprint

ricin
Ricin
Amino Acid Sequence
amino acid sequences
peptides
Amino Acids
Peptides
Paper Chromatography
paper chromatography
Column chromatography
Chromatography
Gel Chromatography
arginine
Arginine
chromatography
Gels
gels
Degradation
amino acids
degradation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Isolation and amino acid sequences of small tryptic peptides from the oxidized ala chain of ricin d. / Kimura, Makoto; Funatsu, Gunki; Funatsu, Masaru.

In: Agricultural and Biological Chemistry, Vol. 41, No. 9, 09.1977, p. 1733-1736.

Research output: Contribution to journalArticle

Kimura, Makoto ; Funatsu, Gunki ; Funatsu, Masaru. / Isolation and amino acid sequences of small tryptic peptides from the oxidized ala chain of ricin d. In: Agricultural and Biological Chemistry. 1977 ; Vol. 41, No. 9. pp. 1733-1736.
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