Isolation and characterization of a family of rat endogenous retroviral sequences

Makoto Nakamuta, Masato Furuich, Keikichi Takahashi, Noriaki Suzuki, Hideya Endo, Mikio Yamamoto

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Abstract

The complete nucleotide sequence of one representative rat genomic unit flanked on both sides with RAL elements, which have structural features specific to retroviral LTRs (1), was determined. The total unit was about 7.5 kbp long, and there was a partial homology to known retroviral sequences in gag, pol, and env regions. The sequence also contained minus- and plus-strand primer binding sites, thereby indicating a retroviral nature in replication. Transcription of the sequence was extensive in tumor cells and was strongly correlated with the state of methylation within 5′ LTRs, which were highly methylated in the normal but not in the tumor state. In functional assays with bacterial chloramphenicol acetyltransferase constructs containing a series of deleted LTRs, there seemed to be both positive and negative cis-acting effector sequences.

Original languageEnglish
Pages (from-to)69-83
Number of pages15
JournalVirus Genes
Volume3
Issue number1
DOIs
Publication statusPublished - Sep 1 1989

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Virology

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