Isolation and Characterization of a Filamentous Phage, Vf33, Specific for Vibrio parahaemolyticus

Hatsumi Taniguchi, Kiyoshi Sato, Midori Ogawa, Takezo Udou, Yasuo Mizuguchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Phage Vf33, a filamentous phage about 1,400 nm long and 7 nm wide, specific for Vibrio parahaemolyticus, was isolated and characterized. The buoyant density of Vf33 in CsCl was 1.292 g/cm3. As with other filamentous phages, the lytic activity of Vf33 was resistant to heating below 80 C and to treatment with diethylether, acetone or methanol but sensitive to chloroform. The nucleic acid of this phage is single-stranded circular DNA 8.4 kb in size. The viral genome was converted to a double-stranded replicative form in the host cell. Among the strains tested, only V, parahaemolyticus strains possessing K38 antigen was sensitive to the phage.

Original languageEnglish
Pages (from-to)327-337
Number of pages11
JournalMICROBIOLOGY and IMMUNOLOGY
Volume28
Issue number3
DOIs
Publication statusPublished - Jan 1 1984

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Vibrio parahaemolyticus
Bacteriophages
Circular DNA
Viral Genome
Single-Stranded DNA
Chloroform
Acetone
Heating
Nucleic Acids
Methanol
Antigens

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Isolation and Characterization of a Filamentous Phage, Vf33, Specific for Vibrio parahaemolyticus. / Taniguchi, Hatsumi; Sato, Kiyoshi; Ogawa, Midori; Udou, Takezo; Mizuguchi, Yasuo.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 28, No. 3, 01.01.1984, p. 327-337.

Research output: Contribution to journalArticle

Taniguchi, Hatsumi ; Sato, Kiyoshi ; Ogawa, Midori ; Udou, Takezo ; Mizuguchi, Yasuo. / Isolation and Characterization of a Filamentous Phage, Vf33, Specific for Vibrio parahaemolyticus. In: MICROBIOLOGY and IMMUNOLOGY. 1984 ; Vol. 28, No. 3. pp. 327-337.
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