TY - JOUR
T1 - Isolation and Characterization of a Filamentous Phage, Vf33, Specific for Vibrio parahaemolyticus
AU - Taniguchi, Hatsumi
AU - Sato, Kiyoshi
AU - Ogawa, Midori
AU - Udou, Takezo
AU - Mizuguchi, Yasuo
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1984
Y1 - 1984
N2 - Phage Vf33, a filamentous phage about 1,400 nm long and 7 nm wide, specific for Vibrio parahaemolyticus, was isolated and characterized. The buoyant density of Vf33 in CsCl was 1.292 g/cm3. As with other filamentous phages, the lytic activity of Vf33 was resistant to heating below 80 C and to treatment with diethylether, acetone or methanol but sensitive to chloroform. The nucleic acid of this phage is single-stranded circular DNA 8.4 kb in size. The viral genome was converted to a double-stranded replicative form in the host cell. Among the strains tested, only V, parahaemolyticus strains possessing K38 antigen was sensitive to the phage.
AB - Phage Vf33, a filamentous phage about 1,400 nm long and 7 nm wide, specific for Vibrio parahaemolyticus, was isolated and characterized. The buoyant density of Vf33 in CsCl was 1.292 g/cm3. As with other filamentous phages, the lytic activity of Vf33 was resistant to heating below 80 C and to treatment with diethylether, acetone or methanol but sensitive to chloroform. The nucleic acid of this phage is single-stranded circular DNA 8.4 kb in size. The viral genome was converted to a double-stranded replicative form in the host cell. Among the strains tested, only V, parahaemolyticus strains possessing K38 antigen was sensitive to the phage.
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U2 - 10.1111/j.1348-0421.1984.tb00684.x
DO - 10.1111/j.1348-0421.1984.tb00684.x
M3 - Article
C2 - 6738383
AN - SCOPUS:0021252514
VL - 28
SP - 327
EP - 337
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 3
ER -