Isolation and characterization of a new facultatively autotrophic hydrogen-oxidizing Betaproteobacterium, Hydrogenophaga sp. AH-24

Ki Seok Yoon, Natsuki Tsukada, Yukiko Sakai, Masaharu Ishii, Yasuo Igarashi, Hirofumi Nishihara

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A hydrogen-oxidizing bacterium strain AH-24 was isolated, which was classified in the genus Hydrogenophaga, based on the 16S rRNA gene sequence. The isolate possessed a typical yellow pigment of Hydrogenophaga species. Its closest relative was Hydrogenophaga pseudoflava, but the assimilation profile of sugar compounds resembled that of no species of Hydrogenophaga. The optimum temperature and pH for autotrophic growth were, respectively, 33-35°C and 7.0. Most hydrogenase activity (benzyl viologen reducing activity) was localized in the membrane fraction (MF), but NAD(P)-reducing hydrogenase activity was detected in neither the membrane nor the soluble fractions. Cytochromes b 561 and c551 were present in MF; both were reduced when hydrogen was supplied to the oxidized MF, suggesting involvement in respiratory H2 oxidation as electron carriers. Cytochrome b561 was inferred to function as the redox partner of the membrane-bound hydrogenase.

Original languageEnglish
Pages (from-to)94-100
Number of pages7
JournalFEMS microbiology letters
Volume278
Issue number1
DOIs
Publication statusPublished - Jan 1 2008

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Comamonadaceae
Betaproteobacteria
Hydrogen
Membranes
Hydrogenase
hydrogen dehydrogenase
Benzyl Viologen
Autotrophic Processes
rRNA Genes
Oxidation-Reduction
Electrons
Bacteria
Temperature

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Isolation and characterization of a new facultatively autotrophic hydrogen-oxidizing Betaproteobacterium, Hydrogenophaga sp. AH-24. / Yoon, Ki Seok; Tsukada, Natsuki; Sakai, Yukiko; Ishii, Masaharu; Igarashi, Yasuo; Nishihara, Hirofumi.

In: FEMS microbiology letters, Vol. 278, No. 1, 01.01.2008, p. 94-100.

Research output: Contribution to journalArticle

Yoon, Ki Seok ; Tsukada, Natsuki ; Sakai, Yukiko ; Ishii, Masaharu ; Igarashi, Yasuo ; Nishihara, Hirofumi. / Isolation and characterization of a new facultatively autotrophic hydrogen-oxidizing Betaproteobacterium, Hydrogenophaga sp. AH-24. In: FEMS microbiology letters. 2008 ; Vol. 278, No. 1. pp. 94-100.
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