Abstract
During an entomological surveillance for arthropod-borne viruses in the Philippines, we isolated a previously unrecognized virus from female Armigeres spp. mosquitoes. Whole-genome sequencing, genetic characterization and phylogenetic analysis revealed that the isolated virus, designated Armigeres iflavirus (ArIFV), is a novel member of the iflaviruses (genus Iflavirus, family Iflaviridae) and phylogenetically related to Moku virus, Hubei odonate virus 4, slow bee paralysis virus and Graminella nigrifrons virus 1. To our knowledge, this is the first successful isolation of iflavirus from a dipteran insect. Spherical ArIFV particles of approximately 30nm in diameter contained at least three major structural proteins. ArIFV multiplied to high titres (~109 p.f.u. ml-1) and formed clear plaques in a mosquito cell line, C6/36. Our findings provide new insights into the infection mechanism, genetic diversity and evolution of the Iflaviridae family.
| Original language | English |
|---|---|
| Article number | 000929 |
| Pages (from-to) | 2876-2881 |
| Number of pages | 6 |
| Journal | Journal of General Virology |
| Volume | 98 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 1 2017 |
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All Science Journal Classification (ASJC) codes
- Virology
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Isolation and characterization of a new iflavirus from armigeres spp. Mosquitoes in the Philippines. / Kobayashi, Daisuke; Isawa, Haruhiko; Fujita, Ryosuke; Murota, Katsunori; Itokawa, Kentaro; Higa, Yukiko; Katayama, Yukie; Sasaki, Toshinori; Mizutani, Tetsuya; Iwanaga, Shiroh; Ohta, Nobuo; Garcia-Bertuso, Arlene; Sawabe, Kyoko.
In: Journal of General Virology, Vol. 98, No. 11, 000929, 01.11.2017, p. 2876-2881.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of a new iflavirus from armigeres spp. Mosquitoes in the Philippines
AU - Kobayashi, Daisuke
AU - Isawa, Haruhiko
AU - Fujita, Ryosuke
AU - Murota, Katsunori
AU - Itokawa, Kentaro
AU - Higa, Yukiko
AU - Katayama, Yukie
AU - Sasaki, Toshinori
AU - Mizutani, Tetsuya
AU - Iwanaga, Shiroh
AU - Ohta, Nobuo
AU - Garcia-Bertuso, Arlene
AU - Sawabe, Kyoko
PY - 2017/11/1
Y1 - 2017/11/1
N2 - During an entomological surveillance for arthropod-borne viruses in the Philippines, we isolated a previously unrecognized virus from female Armigeres spp. mosquitoes. Whole-genome sequencing, genetic characterization and phylogenetic analysis revealed that the isolated virus, designated Armigeres iflavirus (ArIFV), is a novel member of the iflaviruses (genus Iflavirus, family Iflaviridae) and phylogenetically related to Moku virus, Hubei odonate virus 4, slow bee paralysis virus and Graminella nigrifrons virus 1. To our knowledge, this is the first successful isolation of iflavirus from a dipteran insect. Spherical ArIFV particles of approximately 30nm in diameter contained at least three major structural proteins. ArIFV multiplied to high titres (~109 p.f.u. ml-1) and formed clear plaques in a mosquito cell line, C6/36. Our findings provide new insights into the infection mechanism, genetic diversity and evolution of the Iflaviridae family.
AB - During an entomological surveillance for arthropod-borne viruses in the Philippines, we isolated a previously unrecognized virus from female Armigeres spp. mosquitoes. Whole-genome sequencing, genetic characterization and phylogenetic analysis revealed that the isolated virus, designated Armigeres iflavirus (ArIFV), is a novel member of the iflaviruses (genus Iflavirus, family Iflaviridae) and phylogenetically related to Moku virus, Hubei odonate virus 4, slow bee paralysis virus and Graminella nigrifrons virus 1. To our knowledge, this is the first successful isolation of iflavirus from a dipteran insect. Spherical ArIFV particles of approximately 30nm in diameter contained at least three major structural proteins. ArIFV multiplied to high titres (~109 p.f.u. ml-1) and formed clear plaques in a mosquito cell line, C6/36. Our findings provide new insights into the infection mechanism, genetic diversity and evolution of the Iflaviridae family.
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UR - http://www.scopus.com/inward/citedby.url?scp=85033558442&partnerID=8YFLogxK
U2 - 10.1099/jgv.0.000929
DO - 10.1099/jgv.0.000929
M3 - Article
C2 - 29048274
AN - SCOPUS:85033558442
VL - 98
SP - 2876
EP - 2881
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 11
M1 - 000929
ER -