Isolation and characterization of a new iflavirus from armigeres spp. Mosquitoes in the Philippines

Daisuke Kobayashi, Haruhiko Isawa, Ryosuke Fujita, Katsunori Murota, Kentaro Itokawa, Yukiko Higa, Yukie Katayama, Toshinori Sasaki, Tetsuya Mizutani, Shiroh Iwanaga, Nobuo Ohta, Arlene Garcia-Bertuso, Kyoko Sawabe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

During an entomological surveillance for arthropod-borne viruses in the Philippines, we isolated a previously unrecognized virus from female Armigeres spp. mosquitoes. Whole-genome sequencing, genetic characterization and phylogenetic analysis revealed that the isolated virus, designated Armigeres iflavirus (ArIFV), is a novel member of the iflaviruses (genus Iflavirus, family Iflaviridae) and phylogenetically related to Moku virus, Hubei odonate virus 4, slow bee paralysis virus and Graminella nigrifrons virus 1. To our knowledge, this is the first successful isolation of iflavirus from a dipteran insect. Spherical ArIFV particles of approximately 30nm in diameter contained at least three major structural proteins. ArIFV multiplied to high titres (~109 p.f.u. ml-1) and formed clear plaques in a mosquito cell line, C6/36. Our findings provide new insights into the infection mechanism, genetic diversity and evolution of the Iflaviridae family.

Original languageEnglish
Article number000929
Pages (from-to)2876-2881
Number of pages6
JournalJournal of General Virology
Volume98
Issue number11
DOIs
Publication statusPublished - Nov 1 2017

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Philippines
Culicidae
Viruses
Arboviruses
Molecular Evolution
Bees
Paralysis
Insects
Genome
Cell Line
Infection
Proteins

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Kobayashi, D., Isawa, H., Fujita, R., Murota, K., Itokawa, K., Higa, Y., ... Sawabe, K. (2017). Isolation and characterization of a new iflavirus from armigeres spp. Mosquitoes in the Philippines. Journal of General Virology, 98(11), 2876-2881. [000929]. https://doi.org/10.1099/jgv.0.000929

Isolation and characterization of a new iflavirus from armigeres spp. Mosquitoes in the Philippines. / Kobayashi, Daisuke; Isawa, Haruhiko; Fujita, Ryosuke; Murota, Katsunori; Itokawa, Kentaro; Higa, Yukiko; Katayama, Yukie; Sasaki, Toshinori; Mizutani, Tetsuya; Iwanaga, Shiroh; Ohta, Nobuo; Garcia-Bertuso, Arlene; Sawabe, Kyoko.

In: Journal of General Virology, Vol. 98, No. 11, 000929, 01.11.2017, p. 2876-2881.

Research output: Contribution to journalArticle

Kobayashi, D, Isawa, H, Fujita, R, Murota, K, Itokawa, K, Higa, Y, Katayama, Y, Sasaki, T, Mizutani, T, Iwanaga, S, Ohta, N, Garcia-Bertuso, A & Sawabe, K 2017, 'Isolation and characterization of a new iflavirus from armigeres spp. Mosquitoes in the Philippines', Journal of General Virology, vol. 98, no. 11, 000929, pp. 2876-2881. https://doi.org/10.1099/jgv.0.000929
Kobayashi, Daisuke ; Isawa, Haruhiko ; Fujita, Ryosuke ; Murota, Katsunori ; Itokawa, Kentaro ; Higa, Yukiko ; Katayama, Yukie ; Sasaki, Toshinori ; Mizutani, Tetsuya ; Iwanaga, Shiroh ; Ohta, Nobuo ; Garcia-Bertuso, Arlene ; Sawabe, Kyoko. / Isolation and characterization of a new iflavirus from armigeres spp. Mosquitoes in the Philippines. In: Journal of General Virology. 2017 ; Vol. 98, No. 11. pp. 2876-2881.
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