Isolation and Characterization of a Wound-inducible Ribonuclease from Nicotiana glutinosa Leaves

Tohru Kariu, Kazunari Sano, Hidetoshi Shimokawa, Riyoko Itoh, Nobuyuki Yamasaki, Makoto Kimura

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A wound-inducible ribonuclease (RNase NW) was purified from leaves of Nicotiana glutinosa. The purified RNase NW has an optimum pH around 5 and 7, and its base specificity is suggested based on the relative rates of hydrolysis of homopolyribonucleotides to be a preference for guanine base. The complete amino acid sequence of RNase NW was deduced by a combination of protein and cDNA sequencings. The cDNA sequence includes the entire coding sequence for a polypeptide with 229 amino acids including a putative secretion signal peptide at the N-terminus composed of 25 amino acids. The amino acids identified by the protein chemical methods are unambiguously localized within the deduced amino acid sequence from the cDNA sequence. Comparison of the sequence of RNase NW with those of other known plant RNases showed that it was identical except for eight residues to that of N. alata RNase NE, which is present in the style and pollen under normal conditions and is induced in roots in response to phosphate starvation [Dodds et al., Plant. Mol. Biol., 31, 227-238 (1996)]. RNase NW shows considerable sequence similarity to other known RNases, sharing 57% to 84% identical residues. Northern blot analysis using an RNase NW cDNA fragment as a probe showed that the RNase NW transcript was not detected in leaves without wounding, but it was induced within 4 h after wounding and then gradually decreased during 20 h.

Original languageEnglish
Pages (from-to)1144-1151
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number6
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

Amino Acids
Ribonucleases
Complementary DNA
Amino Acid Sequence
Protein Sequence Analysis
Guanine
Protein Sorting Signals
Starvation
Pollen
Nicotiana wound-inducible ribonuclease
Northern Blotting
Tobacco
Hydrolysis
Proteins
Phosphates
Peptides
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Isolation and Characterization of a Wound-inducible Ribonuclease from Nicotiana glutinosa Leaves. / Kariu, Tohru; Sano, Kazunari; Shimokawa, Hidetoshi; Itoh, Riyoko; Yamasaki, Nobuyuki; Kimura, Makoto.

In: Bioscience, Biotechnology and Biochemistry, Vol. 62, No. 6, 01.01.1998, p. 1144-1151.

Research output: Contribution to journalArticle

Kariu, Tohru ; Sano, Kazunari ; Shimokawa, Hidetoshi ; Itoh, Riyoko ; Yamasaki, Nobuyuki ; Kimura, Makoto. / Isolation and Characterization of a Wound-inducible Ribonuclease from Nicotiana glutinosa Leaves. In: Bioscience, Biotechnology and Biochemistry. 1998 ; Vol. 62, No. 6. pp. 1144-1151.
@article{57573b34dfd24d98a15ca5c873b471b4,
title = "Isolation and Characterization of a Wound-inducible Ribonuclease from Nicotiana glutinosa Leaves",
abstract = "A wound-inducible ribonuclease (RNase NW) was purified from leaves of Nicotiana glutinosa. The purified RNase NW has an optimum pH around 5 and 7, and its base specificity is suggested based on the relative rates of hydrolysis of homopolyribonucleotides to be a preference for guanine base. The complete amino acid sequence of RNase NW was deduced by a combination of protein and cDNA sequencings. The cDNA sequence includes the entire coding sequence for a polypeptide with 229 amino acids including a putative secretion signal peptide at the N-terminus composed of 25 amino acids. The amino acids identified by the protein chemical methods are unambiguously localized within the deduced amino acid sequence from the cDNA sequence. Comparison of the sequence of RNase NW with those of other known plant RNases showed that it was identical except for eight residues to that of N. alata RNase NE, which is present in the style and pollen under normal conditions and is induced in roots in response to phosphate starvation [Dodds et al., Plant. Mol. Biol., 31, 227-238 (1996)]. RNase NW shows considerable sequence similarity to other known RNases, sharing 57{\%} to 84{\%} identical residues. Northern blot analysis using an RNase NW cDNA fragment as a probe showed that the RNase NW transcript was not detected in leaves without wounding, but it was induced within 4 h after wounding and then gradually decreased during 20 h.",
author = "Tohru Kariu and Kazunari Sano and Hidetoshi Shimokawa and Riyoko Itoh and Nobuyuki Yamasaki and Makoto Kimura",
year = "1998",
month = "1",
day = "1",
doi = "10.1271/bbb.62.1144",
language = "English",
volume = "62",
pages = "1144--1151",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "6",

}

TY - JOUR

T1 - Isolation and Characterization of a Wound-inducible Ribonuclease from Nicotiana glutinosa Leaves

AU - Kariu, Tohru

AU - Sano, Kazunari

AU - Shimokawa, Hidetoshi

AU - Itoh, Riyoko

AU - Yamasaki, Nobuyuki

AU - Kimura, Makoto

PY - 1998/1/1

Y1 - 1998/1/1

N2 - A wound-inducible ribonuclease (RNase NW) was purified from leaves of Nicotiana glutinosa. The purified RNase NW has an optimum pH around 5 and 7, and its base specificity is suggested based on the relative rates of hydrolysis of homopolyribonucleotides to be a preference for guanine base. The complete amino acid sequence of RNase NW was deduced by a combination of protein and cDNA sequencings. The cDNA sequence includes the entire coding sequence for a polypeptide with 229 amino acids including a putative secretion signal peptide at the N-terminus composed of 25 amino acids. The amino acids identified by the protein chemical methods are unambiguously localized within the deduced amino acid sequence from the cDNA sequence. Comparison of the sequence of RNase NW with those of other known plant RNases showed that it was identical except for eight residues to that of N. alata RNase NE, which is present in the style and pollen under normal conditions and is induced in roots in response to phosphate starvation [Dodds et al., Plant. Mol. Biol., 31, 227-238 (1996)]. RNase NW shows considerable sequence similarity to other known RNases, sharing 57% to 84% identical residues. Northern blot analysis using an RNase NW cDNA fragment as a probe showed that the RNase NW transcript was not detected in leaves without wounding, but it was induced within 4 h after wounding and then gradually decreased during 20 h.

AB - A wound-inducible ribonuclease (RNase NW) was purified from leaves of Nicotiana glutinosa. The purified RNase NW has an optimum pH around 5 and 7, and its base specificity is suggested based on the relative rates of hydrolysis of homopolyribonucleotides to be a preference for guanine base. The complete amino acid sequence of RNase NW was deduced by a combination of protein and cDNA sequencings. The cDNA sequence includes the entire coding sequence for a polypeptide with 229 amino acids including a putative secretion signal peptide at the N-terminus composed of 25 amino acids. The amino acids identified by the protein chemical methods are unambiguously localized within the deduced amino acid sequence from the cDNA sequence. Comparison of the sequence of RNase NW with those of other known plant RNases showed that it was identical except for eight residues to that of N. alata RNase NE, which is present in the style and pollen under normal conditions and is induced in roots in response to phosphate starvation [Dodds et al., Plant. Mol. Biol., 31, 227-238 (1996)]. RNase NW shows considerable sequence similarity to other known RNases, sharing 57% to 84% identical residues. Northern blot analysis using an RNase NW cDNA fragment as a probe showed that the RNase NW transcript was not detected in leaves without wounding, but it was induced within 4 h after wounding and then gradually decreased during 20 h.

UR - http://www.scopus.com/inward/record.url?scp=0032085519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032085519&partnerID=8YFLogxK

U2 - 10.1271/bbb.62.1144

DO - 10.1271/bbb.62.1144

M3 - Article

C2 - 9692197

AN - SCOPUS:0032085519

VL - 62

SP - 1144

EP - 1151

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 6

ER -