Isolation and characterization of alkane-utilizing Nocardioides sp. strain CF8

Natsuko Hamamura, Daniel J. Arp

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A butane-utilizing bacterial strain CF8 was isolated and identified as a member of the genus Nocardioides from chemotaxonomic and 16S rDNA sequence analysis. Strain CF8 grew on alkanes ranging from C2 to C16 in addition to butane and various other substrates including primary alcohols, carboxylic acids, and phenol. Butane degradation by strain CF8 was inactivated by light, a specific inactivator of copper-containing monooxygenases. The unique thermal aggregation phenomenon of acetylene-binding polypeptides was also observed for strain CF8. These results suggest that butane monooxygenase in strain CF8 is a third example of the copper-containing monooxygenases previously described in ammonia oxidizers and methanotrophs. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalFEMS microbiology letters
Volume186
Issue number1
DOIs
Publication statusPublished - May 1 2000

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Alkanes
Mixed Function Oxygenases
Copper
Acetylene
Carboxylic Acids
Ribosomal DNA
Phenol
Ammonia
Sequence Analysis
Hot Temperature
Alcohols
Light
Peptides
butane

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Isolation and characterization of alkane-utilizing Nocardioides sp. strain CF8. / Hamamura, Natsuko; Arp, Daniel J.

In: FEMS microbiology letters, Vol. 186, No. 1, 01.05.2000, p. 21-26.

Research output: Contribution to journalArticle

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