Abstract
We determined a complete nucleotide sequence of an activated form of the c‐H‐ras‐1 proto‐oncogene cloned from the human cell line (QG56), using the DNA transfection technique and NIH3T3 cells as recipients. This cell line was established from a squamous‐cell lung carcinoma of a Japanese patient, and the activated gene had 2 nucleotide substitutions. One substitution of a thymidine for an adenosine was found at position 1069 of the 2898 nucleotide sequence in a restriction endonuclease (Sacl) fragment, which corresponds to the second base of the 61 st codon of the gene encoding P 21 protein. This nucleotide replacement was assumed to be responsible for the transforming activity. Another substitution of a guanosine for an adenosine which was detected at position 746 in the first intron was thought to be a genetic polymorphism unassociated with the transforming activity. Comparison of the various lengths of restricted fragments suggested that the activity was markedly influenced by certain sequences flanking the c‐H‐ras‐1 gene.
| Original language | English |
|---|---|
| Pages (from-to) | 809-812 |
| Number of pages | 4 |
| Journal | International Journal of Cancer |
| Volume | 35 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 15 1985 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
Cite this
Isolation and characterization of an activated c‐H‐ras‐1 gene from a squamous‐cell lung carcinoma cell line. / Kagimoto, Masaaki; Miyoshi, Jun; Tashiro, Kosuke; Naito, Yasushi; Sakaki, Yoshiyuki; Sueishi, Katsuo; Tanaka, Kenzo; Imamura, Takashi.
In: International Journal of Cancer, Vol. 35, No. 6, 15.06.1985, p. 809-812.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of an activated c‐H‐ras‐1 gene from a squamous‐cell lung carcinoma cell line
AU - Kagimoto, Masaaki
AU - Miyoshi, Jun
AU - Tashiro, Kosuke
AU - Naito, Yasushi
AU - Sakaki, Yoshiyuki
AU - Sueishi, Katsuo
AU - Tanaka, Kenzo
AU - Imamura, Takashi
PY - 1985/6/15
Y1 - 1985/6/15
N2 - We determined a complete nucleotide sequence of an activated form of the c‐H‐ras‐1 proto‐oncogene cloned from the human cell line (QG56), using the DNA transfection technique and NIH3T3 cells as recipients. This cell line was established from a squamous‐cell lung carcinoma of a Japanese patient, and the activated gene had 2 nucleotide substitutions. One substitution of a thymidine for an adenosine was found at position 1069 of the 2898 nucleotide sequence in a restriction endonuclease (Sacl) fragment, which corresponds to the second base of the 61 st codon of the gene encoding P 21 protein. This nucleotide replacement was assumed to be responsible for the transforming activity. Another substitution of a guanosine for an adenosine which was detected at position 746 in the first intron was thought to be a genetic polymorphism unassociated with the transforming activity. Comparison of the various lengths of restricted fragments suggested that the activity was markedly influenced by certain sequences flanking the c‐H‐ras‐1 gene.
AB - We determined a complete nucleotide sequence of an activated form of the c‐H‐ras‐1 proto‐oncogene cloned from the human cell line (QG56), using the DNA transfection technique and NIH3T3 cells as recipients. This cell line was established from a squamous‐cell lung carcinoma of a Japanese patient, and the activated gene had 2 nucleotide substitutions. One substitution of a thymidine for an adenosine was found at position 1069 of the 2898 nucleotide sequence in a restriction endonuclease (Sacl) fragment, which corresponds to the second base of the 61 st codon of the gene encoding P 21 protein. This nucleotide replacement was assumed to be responsible for the transforming activity. Another substitution of a guanosine for an adenosine which was detected at position 746 in the first intron was thought to be a genetic polymorphism unassociated with the transforming activity. Comparison of the various lengths of restricted fragments suggested that the activity was markedly influenced by certain sequences flanking the c‐H‐ras‐1 gene.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021881598&partnerID=8YFLogxK
U2 - 10.1002/ijc.2910350618
DO - 10.1002/ijc.2910350618
M3 - Article
C2 - 4008102
AN - SCOPUS:0021881598
VL - 35
SP - 809
EP - 812
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 6
ER -