Isolation and characterization of cDNA and genomic promoter region for a heat shock protein 30 from Aspergillus nidulans

Takahiro Kusakabe, Katsumi Koga, Yasushi Sugimoto

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7 Citations (Scopus)

Abstract

A cDNA encoding for a heat shock protein 30 (HSP30) of Aspergillus nidulans and the promoter region of its gene were analyzed for their primary structures. The promoter region had no heat shock element but possessed three inverted repeat sequences. Northern blot hybridization indicated that the expression of the HSP30 gene was high at a normal temperature and was slightly accelerated at elevated temperatures in A. nidulans cells. Although the deduced amino acid sequence of the A. nidulans HSP30 had a domain highly conserved among other small HSPs from different species, it showed a sequence homology of only 42% even in comparison with the most closely related molecular species, Neurospora crassa HSP30. These findings suggest that the present HSP30 belongs to a novel subfamily of low-molecular-weight HSPs.

Original languageEnglish
Pages (from-to)555-558
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1219
Issue number2
DOIs
Publication statusPublished - Oct 18 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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