Isolation and characterization of cDNA and genomic sequences for mouse O6-methylguanine-DNA methyltransferase

Akiko Shiraishi, Sakumi Kunihiko, Yoshimichi Nakatsu, Hiroshi Hayakawa, Mutsuo Sekiguchi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

An enzyme, O6-methylguanine-DNA methyltransferase, is present in various organisms and plays an important role in repair of DNA damaged by alkylating agents. The enzyme transfers methyl groups from O6-methylguanine and other methylated moieties of the DNA to its own molecule. As a first step to construct animal models with altered levels of the enzyme activity, we cloned cDNA and genomic DNA sequences for mouse methyltransferase and elucidated their structures. The nucleotide sequence of the cDNA revealed an open reading frame comprising 211 amino acid residues. The mol. wt of mouse O6-methylguanine-DNA methyltransferase, calculated from the predicted amino acid sequence, was 22 400, and the methyltransferase protein of this size was present when the cDNA was expressed in methyltransferasedeficient human cells. The predicted amino acid sequence of the mouse methyltransferase exhibits an intense homology with those of human and bacterial counterparts. Using the cDNA as a probe, part of the mouse gene for methyltransferase was isolated. The gene consisted of at least four exons and spanned > 145 KB. Sequences around the exon/intron junctions for the mouse gene are almost the same as those for the human species.

Original languageEnglish
Pages (from-to)289-296
Number of pages8
JournalCarcinogenesis
Volume13
Issue number2
DOIs
Publication statusPublished - Feb 1 1992

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Methyltransferases
Complementary DNA
DNA
Amino Acid Sequence
Exons
Enzymes
Protein Methyltransferases
Genes
Alkylating Agents
DNA Repair
Introns
Open Reading Frames
Animal Models
O-(6)-methylguanine
Amino Acids

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

Isolation and characterization of cDNA and genomic sequences for mouse O6-methylguanine-DNA methyltransferase. / Shiraishi, Akiko; Kunihiko, Sakumi; Nakatsu, Yoshimichi; Hayakawa, Hiroshi; Sekiguchi, Mutsuo.

In: Carcinogenesis, Vol. 13, No. 2, 01.02.1992, p. 289-296.

Research output: Contribution to journalArticle

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